Albrechtova Jana, Latr Ales, Nedorost Ludovit, Pokluda Robert, Posta Katalin, Vosatka Miroslav
Department of Experimental Plant Biology, Faculty of Science, Charles University in Prague, 12844 Vinicna 5, Czech Republic.
ScientificWorldJournal. 2012;2012:374091. doi: 10.1100/2012/374091. Epub 2012 Apr 30.
The aim of this paper was to test the use of dual microbial inoculation with mycorrhizal and saprotrophic fungi in onion cultivation to enhance yield while maintaining or improving the nutritional quality of onion bulbs. Treatments were two-factorial: (1) arbuscular mycorrhizal fungi (AMF): the mix corresponding to fungal part of commercial product Symbivit (Glomus etunicatum, G. microaggregatum, G. intraradices, G. claroideum, G. mosseae, and G. geosporum) (M1) or the single-fungus inoculum of G. intraradices BEG140 (M2) and (2) bark chips preinoculated with saprotrophic fungi (mix of Gymnopilus sp., Agrocybe praecox, and Marasmius androsaceus) (S). The growth response of onion was the highest for the M1 mix treatment, reaching nearly 100% increase in bulb fresh weight. The effectiveness of dual inoculation was proved by more than 50% increase. We observed a strong correlation (r = 0.83) between the growth response of onion bulbs and AM colonization. All inoculation treatments but the single-fungus one enhanced significantly the total antioxidant capacity of bulb biomass, was the highest values being found for M1, S + M1, and S + M2. We observed some induced enhancement of the contents of mineral elements in bulb tissue (Mg and K contents for the M2 and M2, S, and S + M2 treatments, resp.).
本文的目的是测试在洋葱种植中使用菌根真菌和腐生真菌进行双重微生物接种，以提高产量，同时保持或改善洋葱鳞茎的营养品质。试验处理为双因素：（1）丛枝菌根真菌（AMF）：对应于商业产品Symbivit真菌部分的混合物（埃氏球囊霉、微小球囊霉、根内球囊霉、明球囊霉、摩西球囊霉和地球囊霉）（M1）或根内球囊霉BEG140的单菌接种物（M2）；（2）预先接种腐生真菌的树皮屑（裸盖菇属、早生田头菇和红根须腹菌的混合物）（S）。洋葱对M1混合处理的生长响应最高，鳞茎鲜重增加近100%。双重接种的有效性通过增加50%以上得到证明。我们观察到洋葱鳞茎的生长响应与AM定殖之间存在很强的相关性（r = 0.83）。除单菌处理外，所有接种处理均显著提高了鳞茎生物量的总抗氧化能力，其中M1、S + M1和S + M2的值最高。我们观察到鳞茎组织中矿质元素含量有一些诱导性增加（分别为M2以及M2、S和S + M2处理的Mg和K含量）。
