Rodrigues P, Venâncio A, Lima N
CIMO/School of Agriculture of the Polytechnic Institute of Bragança, Campus de Santa Apolónia, 5301-855 Bragança, Portugal.
ScientificWorldJournal. 2012;2012:471926. doi: 10.1100/2012/471926. Epub 2012 Apr 30.
Aflatoxin contamination of nuts is an increasing concern to the consumer's health. Portugal is a big producer of almonds, but there is no scientific knowledge on the safety of those nuts, in terms of mycotoxins. The aim of this paper was to study the incidence of aflatoxigenic fungi and aflatoxin contamination of 21 samples of Portuguese almonds, and its evolution throughout the various stages of production. All fungi belonging to Aspergillus section Flavi were identified and tested for their aflatoxigenic ability. Almond samples were tested for aflatoxin contamination by HPLC-fluorescence. In total, 352 fungi belonging to Aspergillus section Flavi were isolated from Portuguese almonds: 127 were identified as A. flavus (of which 28% produced aflatoxins B), 196 as typical or atypical A. parasiticus (all producing aflatoxins B and G), and 29 as A. tamarii (all nonaflatoxigenic). Aflatoxins were detected in only one sample at 4.97 μg/kg.
坚果中的黄曲霉毒素污染日益引起消费者对健康的担忧。葡萄牙是杏仁的主要生产国，但就霉菌毒素而言，对于这些坚果的安全性尚无科学认识。本文的目的是研究21份葡萄牙杏仁样品中黄曲霉产毒真菌的发生率和黄曲霉毒素污染情况，以及其在生产各阶段的变化。对所有属于黄曲霉组的真菌进行了鉴定，并测试了它们的产毒能力。通过高效液相色谱 - 荧光法对杏仁样品进行黄曲霉毒素污染检测。从葡萄牙杏仁中总共分离出352株属于黄曲霉组的真菌：127株被鉴定为黄曲霉（其中28%产生黄曲霉毒素B），196株为典型或非典型寄生曲霉（均产生黄曲霉毒素B和G），29株为塔宾曲霉（均不产毒）。仅在一个样品中检测到黄曲霉毒素，含量为4.97μg/kg。
