Suzuki Y, Saito H, Kasanuki J, Kishimoto T, Tamura Y, Yoshida S
Second Department of Internal Medicine, Chiba University School of Medicine, Japan.
Life Sci. 1990;47(24):2193-7. doi: 10.1016/0024-3205(90)90149-l.
In the present study, we compared the potency of interleukin 6 production in peripheral blood mononuclear leukocytes between paired patients with active stage and inactive stage of inflammatory bowel disease. Subjects included nine patients with ulcerative colitis, ten patients with Crohn's disease and sex-matched nine healthy volunteers. Mononuclear leukocytes were stimulated with concanavalin A for 24 h to induce interleukin 6 production. Interleukin 6 content in the culture medium was assayed by using specific ELISA and interleukin 6 dependent cell line MH-60. Interleukin 6 production was found to be significantly increased in mononuclear leukocytes from both active ulcerative colitis and Crohn's disease as compared to that from control subjects. There was no significant difference in interleukin 6 production between ulcerative colitis and Crohn's disease. The potency of interleukin 6 production was returned to the control level when the diseases became inactive. The present results, therefore, may indicate some important role of interleukin 6 in the pathogenesis of inflammatory bowel disease and also the potency of interleukin 6 production in mononuclear leukocytes can be an indicator of the activity of inflammatory bowel disease.
在本研究中，我们比较了炎症性肠病活动期和非活动期配对患者外周血单个核白细胞产生白细胞介素6的能力。研究对象包括9例溃疡性结肠炎患者、10例克罗恩病患者以及性别匹配的9名健康志愿者。用刀豆蛋白A刺激单个核白细胞24小时以诱导白细胞介素6的产生。通过使用特异性酶联免疫吸附测定法（ELISA）和依赖白细胞介素6的细胞系MH-60来检测培养基中的白细胞介素6含量。结果发现，与对照组相比，活动期溃疡性结肠炎和克罗恩病患者的单个核白细胞中白细胞介素6的产生均显著增加。溃疡性结肠炎和克罗恩病患者之间白细胞介素6的产生没有显著差异。当疾病进入非活动期时，白细胞介素6的产生能力恢复到对照水平。因此，目前的结果可能表明白细胞介素6在炎症性肠病发病机制中具有一些重要作用，并且单个核白细胞中白细胞介素6的产生能力可以作为炎症性肠病活动的一个指标。
