一株西班牙产产酸克雷伯菌临床分离株中新型 IMP-28 金属β-内酰胺酶的特性研究。
Characterization of a novel IMP-28 metallo-β-lactamase from a Spanish Klebsiella oxytoca clinical isolate.
机构信息
Servicio de Microbiología-INIBIC, Complejo Hospitalario Universitario A Coruña, A Coruña, Spain.
出版信息
Antimicrob Agents Chemother. 2012 Aug;56(8):4540-3. doi: 10.1128/AAC.00776-12. Epub 2012 Jun 5.
Abstract
An isolate of Klebsiella oxytoca carrying a novel IMP metallo-β-lactamase was discovered in Madrid, Spain. The bla(IMP-28) gene is part of a chromosomally located class I integron. The IMP-28 k(cat)/K(m) values for ampicillin, ceftazidime, and cefepime and, to a lesser extent, imipenem and meropenem, are clearly lower than those of IMP-1. The His306Gln mutation may induce important modifications of the L3 loop and thus of substrate accessibility and hydrolysis and be the main reason for this behavior.
摘要
在西班牙马德里发现一株携带新型 IMP 金属β-内酰胺酶的产酸克雷伯菌。bla(IMP-28)基因位于染色体定位的 I 类整合子上。IMP-28 的 k(cat)/K(m)值对氨苄西林、头孢他啶和头孢吡肟，以及在较小程度上对亚胺培南和美罗培南，明显低于 IMP-1。His306Gln 突变可能诱导 L3 环的重要修饰，从而影响底物的可及性和水解，这可能是导致这种行为的主要原因。
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