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Determination of glutathione and glutathione disulfide in biological samples.

作者信息

Anderson M E

出版信息

Methods Enzymol. 1985;113:548-55. doi: 10.1016/s0076-6879(85)13073-9.

DOI:10.1016/s0076-6879(85)13073-9
PMID:4088074
Abstract
摘要

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Determination of glutathione and glutathione disulfide in biological samples.生物样品中谷胱甘肽和谷胱甘肽二硫化物的测定。
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Alkaline hydrolysis of N-ethylmaleimide allows a rapid assay of glutathione disulfide in biological samples.N-乙基马来酰亚胺的碱性水解可用于快速检测生物样品中的谷胱甘肽二硫化物。
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Determination of penicillamine, penicillamine disulfide and penicillamine-glutathione mixed disulfide by high-performance liquid chromatography with electrochemical detection.高效液相色谱-电化学检测法测定青霉胺、二硫化青霉胺及青霉胺-谷胱甘肽混合二硫化物
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Analysis of glutathione, glutathione disulfide, cysteine, homocysteine, and other biological thiols by high-performance liquid chromatography following derivatization by n-(1-pyrenyl)maleimide.经N-(1-芘基)马来酰亚胺衍生化后,用高效液相色谱法分析谷胱甘肽、二硫化谷胱甘肽、半胱氨酸、同型半胱氨酸及其他生物硫醇。
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