Martin J, White I N
MRC Toxicology Unit, Carshalton, Surrey, UK.
J Chromatogr. 1991 Jul 17;568(1):219-25. doi: 10.1016/0378-4347(91)80356-h.
A high-performance liquid chromatographic method utilising fluorimetric detection of oxidised and reduced glutathione, following derivatization with dansyl chloride is described. Dansyl derivatives are separated on an aminopropyl silica column with a methanol-sodium acetate gradient system giving detection limits (signal-to-noise ratio = 2) of 1 pmol. This is in the order of 100-fold more sensitive than established methods based on the ultraviolet detection of dinitrophenylglutathione derivatives. The present procedures have been used to determine oxidised and reduced glutathione in rat lung tissues and in alveolar macrophages.
本文描述了一种高效液相色谱法，该方法利用丹磺酰氯衍生化后，通过荧光检测氧化型和还原型谷胱甘肽。丹磺酰衍生物在氨丙基硅胶柱上，采用甲醇 - 醋酸钠梯度系统进行分离，检测限（信噪比 = 2）为1皮摩尔。这比基于二硝基苯基谷胱甘肽衍生物紫外检测的现有方法灵敏度高约100倍。目前的方法已用于测定大鼠肺组织和肺泡巨噬细胞中的氧化型和还原型谷胱甘肽。
