Huang Huey-Chun, Lien Hsiu-Man, Ke Hui-Ju, Chang Li-Ling, Chen Chia-Chang, Chang Tsong-Min
Department of Medical Laboratory Science and Biotechnology, China Medical University, No, 91 Hsueh-Shih Road, Taichung 40402, Taiwan.
Department of Chemistry, Tung Hai University, No, 181, Section 3, Taichung Port Road, Taichung 40704, Taiwan.
Int J Mol Sci. 2012;13(5):6220-6235. doi: 10.3390/ijms13056220. Epub 2012 May 21.
The purpose of the study was to investigate the antioxidant characteristics of Anisomeles indica methanol extract and the inhibitory effect of ovatodiolide on melanogenesis. In the study, the antioxidant capacities of A. indica methanol extract such as DPPH assay, ABTS radical scavenging assay, reducing capacity and metal ion chelating capacity as well as total phenolic content of the extract were investigated. In addition, the inhibitory effects of ovatodiolide on mushroom tyrosinase, B16F10 intracellular tyrosinase and melanin content were determined spectrophotometrically. Our results revealed that the antioxidant capacities of A. indica methanol extract increased in a dose-dependent pattern. The purified ovatodiolide inhibited mushroom tyrosinase activity (IC(50) = 0.253 mM), the compound also effectively suppressed intracellular tyrosinase activity (IC(50) = 0.469 mM) and decreased the amount of melanin (IC(50) = 0.435 mM) in a dose-dependent manner in B16F10 cells. Our results concluded that A. indica methanol extract displays antioxidant capacities and ovatodiolide purified from the extract inhibited melanogenesis in B16F10 cells. Hence, A. indica methanol extract and ovatodiolide could be applied as a type of dermatological whitening agent in skin care products.
本研究的目的是考察广防风甲醇提取物的抗氧化特性以及卵叶二萜内酯对黑色素生成的抑制作用。在该研究中，对广防风甲醇提取物的抗氧化能力进行了考察，包括DPPH法、ABTS自由基清除法、还原能力和金属离子螯合能力，以及提取物的总酚含量。此外，采用分光光度法测定了卵叶二萜内酯对蘑菇酪氨酸酶、B16F10细胞内酪氨酸酶及黑色素含量的抑制作用。我们的结果表明，广防风甲醇提取物的抗氧化能力呈剂量依赖性增加。纯化的卵叶二萜内酯抑制蘑菇酪氨酸酶活性（IC(50)=0.253 mM），该化合物还能有效抑制B16F10细胞内酪氨酸酶活性（IC(50)=0.469 mM），并呈剂量依赖性降低黑色素含量（IC(50)=0.435 mM）。我们的结果得出结论，广防风甲醇提取物具有抗氧化能力，从提取物中纯化得到的卵叶二萜内酯可抑制B16F10细胞的黑色素生成。因此，广防风甲醇提取物和卵叶二萜内酯可作为一种皮肤美白剂应用于护肤品中。
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