Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States.
Curr Opin Chem Biol. 2012 Aug;16(3-4):285-91. doi: 10.1016/j.cbpa.2012.05.186. Epub 2012 Jun 4.
Synthetic biology, with its goal of designing biological entities for wide-ranging purposes, remains a field of intensive research interest. However, the vast complexity of biological systems has heretofore rendered rational design prohibitively difficult. As a result, directed evolution remains a valuable tool for synthetic biology, enabling the identification of desired functionalities from large libraries of variants. This review highlights the most recent advances in the use of directed evolution in synthetic biology, focusing on new techniques and applications at the pathway and genome scale.
合成生物学以设计用于广泛目的的生物实体为目标，仍然是一个研究热点。然而，生物系统的巨大复杂性使得合理设计变得非常困难。因此，定向进化仍然是合成生物学的一个有价值的工具，能够从大型变体文库中鉴定出所需的功能。本文综述了定向进化在合成生物学中的最新进展，重点介绍了在途径和基因组水平上的新技术和应用。
