Zhao Ping, He Ying, Chu Yue-feng, Gao Peng-cheng, Zhang Xuan, Zhang Nian-zhang, Zhao Hai-yan, Zhang Ke-shan, Lu Zhong-xin
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Epizootic Diseases of Grazing Animals of Ministry of Agriculture, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
J Vet Med Sci. 2012 Sep;74(9):1109-15. doi: 10.1292/jvms.12-0095. Epub 2012 May 14.
The purpose of this study was to obtain immunogenic proteins and potential proteins of interest that were isolated from Mycoplasma capricolum subsp. capripneumoniae (Mccp) by MALDI-TOF mass spectrometry. One-dimensional SDS-PAGE and two-dimensional gel electrophoresis of whole cell preparation were conducted, and membrane proteome maps were prepared by immunoblotting. One-dimensional SDS-PAGE identified three immunogenic proteins with molecular masses in the range 29-97.2 kDa, two of which were in the membrane protein fraction. After two-dimensional gel electrophoresis, 20 highly immunogenic proteins were identified in the whole cell protein preparation while 9 immunogenic proteins were identified in the membrane protein fraction. This indicated that membrane proteins were the principle immunogenic proteins in Mccp. These proteins may have potential for the development of improved diagnostic tests and possible vaccines.
本研究的目的是通过基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF）从山羊肺炎支原体（Mccp）中获得免疫原性蛋白和潜在的目标蛋白。对全细胞制剂进行了一维SDS-PAGE和二维凝胶电泳，并通过免疫印迹制备了膜蛋白质组图谱。一维SDS-PAGE鉴定出三种分子量在29-97.2 kDa范围内的免疫原性蛋白，其中两种位于膜蛋白组分中。二维凝胶电泳后，在全细胞蛋白制剂中鉴定出20种高度免疫原性蛋白，而在膜蛋白组分中鉴定出9种免疫原性蛋白。这表明膜蛋白是Mccp中的主要免疫原性蛋白。这些蛋白可能具有开发改进诊断测试和潜在疫苗的潜力。
