Department of Genomic Drug Discovery Science, Kyoto University Graduate School of Pharmaceutical Sciences, Sakyo-ku, Kyoto, Japan.
FEBS Lett. 2012 May 21;586(10):1547-54. doi: 10.1016/j.febslet.2012.04.021. Epub 2012 Apr 21.
Synapsins are neuronal phosphoproteins that coat synaptic vesicles and are believed to function in the regulation of neurotransmitter release. The signaling mechanism for short-chain free fatty acid (SCFA)-stimulated NE release was examined using primary-cultured mouse sympathetic cervical ganglion neurons. Pharmacological and knockdown experiments showed that activation of sympathetic neurons by SCFA propionate involves SCFA receptor GPR41 linking to G??-PLC?3-ERK1/2-synapsin 2 signaling. Further, synapsin 2b directly interacts with activated ERK1/2 and can be phosphorylated on serine when SCFA activates sympathetic neurons.
突触素是一种神经元磷酸化蛋白，覆盖在突触小泡上，被认为在神经递质释放的调节中起作用。使用原代培养的小鼠交感颈神经节神经元研究了短链游离脂肪酸（SCFA）刺激去甲肾上腺素（NE）释放的信号转导机制。药理和敲低实验表明，丙酸盐通过 SCFA 受体 GPR41 连接到 G??-PLC?3-ERK1/2-突触素 2 信号，激活交感神经元。此外，当 SCFA 激活交感神经元时，突触素 2b 直接与激活的 ERK1/2 相互作用，并可在丝氨酸上磷酸化。
