Equipe MaCSE, Institut des Sciences Chimiques de Rennes, Université de Rennes 1, CNRS UMR n° 6226, Rennes 35042, France.
ChemSusChem. 2012 Jun;5(6):995-1005. doi: 10.1002/cssc.201100835.
Catalyses of electrode reactions by oxidoreductases or living electroactive bacteria are compared and recent advances reviewed. The relation between the biological and nevertheless inert nature of enzymes and the living machinery of electroactive microbes is discussed. The way these biocatalysts may be electrically contacted to anodes or cathodes is considered with a focus on their immobilization at electrodes and on the issue of time stability of these assemblies. Recent improvements in power output of biofuel cells are reviewed together with applications that have appeared in the literature. This account also reviews new approaches for combining enzymes and living microbes in bioelectrochemical systems such as reproducing microbial metabolisms with enzyme cascades and expressing oxidoreductases on genetically engineered microbes. Finally, the use of surface chemistry for studying the microbe-electrode interface and bioelectrodes with cell organelles, such as mitochondria, or with higher organisms, such as yeasts, are discussed. Some perspectives for future research to extend this field are offered as conclusions.
比较了氧化还原酶或活性电细菌对电极反应的催化作用，并对最近的进展进行了综述。讨论了酶的生物性质和惰性与电活性微生物的活体机械之间的关系。考虑了将这些生物催化剂电接触到阳极或阴极的方式，重点是它们在电极上的固定化以及这些组件的时间稳定性问题。本文还回顾了生物燃料电池功率输出的最新改进以及文献中出现的应用。本综述还回顾了在生物电化学系统中结合酶和活体微生物的新方法，例如通过酶级联反应再现微生物代谢以及在基因工程微生物上表达氧化还原酶。最后，讨论了表面化学在研究微生物-电极界面和带有细胞器（如线粒体）或高等生物（如酵母）的生物电极中的应用。作为结论，提供了一些扩展该领域的未来研究展望。
