Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
J Virol Methods. 2012 Oct;185(1):24-31. doi: 10.1016/j.jviromet.2012.05.021. Epub 2012 Jun 5.
A new modified triple-antigen detection test was developed for the direct detection of the influenza A virus. The nucleoprotein (NP), matrix (M), and non-structural (NS1) proteins were used as target antigens because they are abundant in infected cells. Monoclonal antibodies specific to the NP, M, and NS1 proteins were generated. The antibody pairs were selected and evaluated for their reactivity individually and in combination in the triple-antigen detection using sandwich ELISA. Triple-antigen detection demonstrated a higher sensitivity than individual antigen detection when tested with both the H1N1 and H3N2 influenza A viruses. This was illustrated by the 4-fold lower limit of detection of the triple-antigen test than the individual antigen detection test. The findings demonstrated that the sensitivity of influenza A antigen detection was improved with the triple-antigen detection system as compared to individual antigen detection. Therefore, this technique could be a useful tool for the direct detection of cell-associated influenza A antigen. Furthermore, it could provide a basis for the development of a rapid triple-antigen test for influenza A diagnosis.
一种新的改良型三联抗原检测试验被开发出来，用于直接检测甲型流感病毒。核蛋白（NP）、基质（M）和非结构（NS1）蛋白被用作靶抗原，因为它们在感染细胞中丰富。生成了针对 NP、M 和 NS1 蛋白的单克隆抗体。使用夹心 ELISA 单独和组合对抗体对进行了反应性选择和评估。与 H1N1 和 H3N2 甲型流感病毒一起测试时，三联抗原检测的灵敏度高于单个抗原检测。这表明三联抗原检测的检测下限比单个抗原检测低 4 倍。研究结果表明，与单个抗原检测相比，三联抗原检测系统提高了甲型流感抗原检测的灵敏度。因此，该技术可能是直接检测细胞相关甲型流感抗原的有用工具。此外，它还可以为甲型流感诊断的快速三联抗原检测提供基础。
