Department of Microbiology, Queen Mary Hospital, Hong Kong.
J Virol Methods. 2012 Dec;186(1-2):184-8. doi: 10.1016/j.jviromet.2012.09.001. Epub 2012 Sep 7.
Data on analytical sensitivity of rapid diagnostic assays are important for clinical management of influenza, especially during a pandemic. Four rapid antigen detection assays were compared for detection of pandemic influenza A H1N1 2009, seasonal H1N1 and H3N2 in 96 patients with influenza A infection confirmed by real-time RT-PCR. These rapid antigen tests appeared to have lower sensitivity (55.8%) for the diagnosis of pandemic influenza A H1N1 2009 as compared with seasonal H3N2 (71.0%) or H1N1 (69.4%) influenza infections, a difference that was related to a lower viral load in patients infected with the pandemic influenza A H1N1 2009 virus. The detection limit of these antigen detection tests in clinical specimens was an influenza A M gene copy number of average 1.0×10(7) copies/ml. Significant variations between tests in sensitivity for detection of pandemic influenza A H1N1 2009 (43.4-63.3%) were observed. The Directigen EZ Influenza A+B and the Espline Influenza A+B had comparable sensitivity (63%) and were the most sensitive among the four assays evaluated.
数据的分析灵敏度快速诊断检测对于流感的临床管理，特别是在大流行期间非常重要。四种快速抗原检测方法比较检测的大流行性流感 A (H1N1) 2009 年、季节性 H1N1 和 H3N2 在 96 例患者流感感染确诊的实时 RT-PCR。这些快速抗原检测似乎有较低的灵敏度 (55.8%) 为大流行流感 A (H1N1) 2009 与季节性 H3N2 (71.0%) 或 H1N1 (69.4%) 流感感染，这种差异与较低的病毒载量在患者感染大流行流感 A (H1N1) 2009 病毒。检测限这些抗原检测试验在临床标本中是一个流感病毒 A M 基因拷贝数平均 1.0 × 10(7) 拷贝/ml。在检测大流行流感 A (H1N1) 2009 之间的显著变化 (43.4-63.3%) 的敏感性测试。Directigen EZ 流感 A + B 和 Espline 流感 A + B 有可比的敏感性 (63%) 是最敏感的四个检测评估。
