利用硝酸纤维素膜和磁生物传感器检测两种不同的流感病毒。

Detection of two different influenza A viruses using a nitrocellulose membrane and a magnetic biosensor.

机构信息

Electronics and Telecommunications Research Institute, Daejeon, Republic of Korea.

出版信息

J Immunol Methods. 2011 Feb 28;365(1-2):95-100. doi: 10.1016/j.jim.2010.12.005. Epub 2010 Dec 21.

DOI:10.1016/j.jim.2010.12.005

Abstract

Here we describe a new analytical method for the detection of two influenza A viruses by nitrocellulose membrane and magnetic sensors that employ a special frequency mixing technique. The combination of the nitrocellulose membrane and magnetic bead detection permits a rapid assay procedure and excludes two steps (the development of color and the stop reaction) required for usual immunochemical detection methods such as ELISA. Quantitative virus detection was performed using magnetic beads conjugated with secondary antibody. The results were compared with conventional assay methods and with a dot-blot assay with fluorescence compound (FITC). Under optimum conditions, our new assay procedure is capable of detecting picograms of virus per well. This new method combining the nitrocellulose membrane and magnetic bead detection reduces analytical time and allows stable and repeatable analyses of samples in point-of-care applications.

摘要

在这里，我们描述了一种新的分析方法，用于通过硝酸纤维素膜和磁传感器检测两种流感病毒，该方法采用特殊的频率混合技术。硝酸纤维素膜和磁珠检测的结合允许快速测定程序，并排除了常规免疫化学检测方法（如 ELISA）所需的两个步骤（显色和终止反应）。使用与二级抗体偶联的磁性珠进行定量病毒检测。结果与常规测定方法和带有荧光化合物（FITC）的斑点印迹测定法进行了比较。在最佳条件下，我们的新测定程序能够检测到每个孔皮克级的病毒。这种将硝酸纤维素膜和磁珠检测相结合的新方法缩短了分析时间，并允许在即时护理应用中对样品进行稳定和可重复的分析。