野生型细胞中 dnaQ 错义等位基因的 SOS 诱导和诱变。
SOS induction and mutagenesis by dnaQ missense alleles in wild type cells.
机构信息
University of Medicine and Dentistry of New Jersey - New Jersey Medical School, Department of Microbiology and Molecular Genetics, 225 Warren Street, ICPH-E450V, Newark NJ 07101-1709, United States.
出版信息
Mutat Res. 2012 Jul 1;735(1-2):46-50. doi: 10.1016/j.mrfmmm.2012.05.004. Epub 2012 Jun 4.
Abstract
Mistranslation leads to elevated mutagenesis and replication arrest, both of which are hypothesized to result from the presence of mixed populations of wild type and mistranslated versions of DNA polymerase III subunit proteins. Consistent with this possibility, expression of missense alleles of dnaQ (which codes for the proofreading subunit ɛ) in wild type (dnaQ+) cells is shown to lead to SOS induction as well as mutagenesis. Exposure to sublethal concentrations of streptomycin, an aminoglycoside antibiotic known to promote mistranslation, also leads to SOS induction.
摘要
错译会导致突变率升高和复制停滞，这两者都被假设是由于存在野生型和 DNA 聚合酶 III 亚基蛋白错译版本的混合群体所致。与这种可能性一致的是，在野生型（dnaQ+）细胞中表达错义等位基因 dnaQ（编码校对亚基ɛ）会导致 SOS 诱导和突变。暴露于低浓度的链霉素（一种已知能促进错译的氨基糖苷类抗生素）也会导致 SOS 诱导。
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