Sharma Surendra Kumar, Singh Ajay Pal
Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Haryana, India.
J Acupunct Meridian Stud. 2012 Jun;5(3):112-8. doi: 10.1016/j.jams.2012.03.002. Epub 2012 Apr 10.
In this study, the antioxidative potential of a hydroalcoholic extract of Nardostachys jatamansi (NJE) rhizomes was evaluated by various antioxidant assays, including antioxidant capacity by the phosphomolybdenum method, total antioxidant activity in linoleic acid emulsion systems, 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, hydroxyl radicals, nitric oxide (NO) scavenging, metal chelating and reducing power activity. These various antioxidant activities were compared with standard antioxidants such as butylated hydroxytoluene, tocopherol, catechin, and L-ascorbic acid. Total phenolic and flavonoid content of NJE was also determined by a colorimetric method. The extract exhibited high reduction capability and powerful free radical scavenging, especially against DPPH and superoxide anions as well as a moderate effect on NO. Moreover, the peroxidation inhibiting activity of NJE was demonstrated in the linoleic acid emulsion system. The results obtained in the present study clearly established the antioxidative potency of NJE, which may account for some of the medical claims attributed to this plant.
在本研究中，通过各种抗氧化试验评估了甘松根茎水醇提取物（NJE）的抗氧化潜力，包括用磷钼酸法测定抗氧化能力、在亚油酸乳液体系中测定总抗氧化活性、1,1-二苯基-2-苦基肼（DPPH）、超氧化物、羟基自由基、一氧化氮（NO）清除能力、金属螯合能力和还原能力活性。将这些不同的抗氧化活性与丁基羟基甲苯、生育酚、儿茶素和L-抗坏血酸等标准抗氧化剂进行了比较。还通过比色法测定了NJE的总酚和黄酮含量。该提取物表现出高还原能力和强大的自由基清除能力，尤其是对DPPH和超氧阴离子，对NO也有中等程度的清除作用。此外，在亚油酸乳液体系中证实了NJE的过氧化抑制活性。本研究获得的结果清楚地证实了NJE的抗氧化效力，这可能解释了归因于这种植物的一些医学疗效。
