水貂生长激素cDNA的克隆与序列分析

Cloning and sequence analysis of mink growth hormone cDNA.

作者信息

Harada Y, Tatsumi H, Nakano E, Umezu M

机构信息

Research and Development Division, Kikkoman Corporation, Chiba, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Dec 31;173(3):1200-4. doi: 10.1016/s0006-291x(05)80913-1.

DOI:10.1016/s0006-291x(05)80913-1

PMID:2268323

Abstract

A cDNA clone for mink growth hormone (GH) was isolated from a mink pituitary cDNA library, employing a part of rat growth hormone cDNA sequence as a probe. According to the nucleotide sequence, mature mink GH consists of 190 amino acids with a calculated molecular weight of 21,720. The amino acid sequence homology between the mature region of mink GH and those of pig GH, rat GH, bovine GH and human GH was 98.4%, 93.7%, 89.0% and 66.7%, respectively.

摘要

以大鼠生长激素cDNA序列的一部分为探针，从水貂垂体cDNA文库中分离出了水貂生长激素（GH）的cDNA克隆。根据核苷酸序列，成熟的水貂生长激素由190个氨基酸组成，计算分子量为21,720。水貂生长激素成熟区与猪生长激素、大鼠生长激素、牛生长激素和人生长激素的氨基酸序列同源性分别为98.4%、93.7%、89.0%和66.7%。