Ogi K, Kimura C, Onda H, Arimura A, Fujino M
Tsukuba Research Laboratories, Takeda Chemical Industries Ltd., Ibaraki, Japan.
Biochem Biophys Res Commun. 1990 Dec 31;173(3):1271-9. doi: 10.1016/s0006-291x(05)80924-6.
We have isolated and cloned a cDNA for rat pituitary adenylate cyclase activating polypeptide (PACAP) precursor protein from the brain. The nucleotide and the deduced amino acid sequences revealed that the rat PACAP cDNA encoded a 175 amino acid protein highly homologous to the ovine and human PACAP precursors. The rat PACAP precursor protein included a putative signal peptide at the amino-terminus, a PACAP-related peptide (PRP) of 29 amino acid residues and the rat PACAP of 38 amino acid residues flanked by basic processing sites, and a carboxy-teminus amidation signal for PACAP. Furthermore, the amino acid sequence of the rat PACAP was completely identical to that of the ovine and human PACAP. Northern blot analysis of rat brain RNA revealed an approximately 3.0 kb transcript for the PACAP precursor in rat brain.
我们从大鼠脑中分离并克隆了大鼠垂体腺苷酸环化酶激活多肽（PACAP）前体蛋白的cDNA。核苷酸序列和推导的氨基酸序列显示，大鼠PACAP cDNA编码一种由175个氨基酸组成的蛋白质，与绵羊和人类的PACAP前体高度同源。大鼠PACAP前体蛋白在氨基末端包含一个假定的信号肽、一个由29个氨基酸残基组成的PACAP相关肽（PRP）以及由碱性加工位点侧翼的38个氨基酸残基的大鼠PACAP，还有一个用于PACAP的羧基末端酰胺化信号。此外，大鼠PACAP的氨基酸序列与绵羊和人类的PACAP完全相同。对大鼠脑RNA的Northern印迹分析显示，大鼠脑中PACAP前体有一个约3.0 kb的转录本。
