Okazaki K, Itoh Y, Ogi K, Ohkubo S, Onda H
Discovery Research Laboratories I, Takeda Chemical Industries, Ltd, Ibaraki, Japan.
Peptides. 1995;16(7):1295-9. doi: 10.1016/0196-9781(95)02018-r.
A murine PACAP precursor cDNA was isolated by screening a brain cDNA library. The amino acid sequence of the precursor was highly similar (from 81% to 93% similarity) to its rat, human, and ovine counterparts. The primary structure of murine PACAP is identical with those from sheep, humans, and rats, indicating that the mature PACAP is well conserved among mammals. Northern blot analysis revealed that the approximately 2.4 kb transcript for the PACAP precursor is expressed in murine brain. The verification that murine PACAP is identical to its human counterpart provides a rationale for physiological and pathophysiological studies of PACAP in mice.
通过筛选脑cDNA文库分离出小鼠垂体腺苷酸环化酶激活肽（PACAP）前体cDNA。该前体的氨基酸序列与其大鼠、人类和绵羊的对应序列高度相似（相似性为81%至93%）。小鼠PACAP的一级结构与绵羊、人类和大鼠的相同，表明成熟的PACAP在哺乳动物中高度保守。Northern印迹分析显示，PACAP前体约2.4 kb的转录本在小鼠脑中表达。小鼠PACAP与其人类对应物相同的验证为在小鼠中进行PACAP的生理和病理生理研究提供了理论依据。
