Key Laboratory for NeuroInformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Life Sci. 2012 Jun 27;90(25-26):1010-4. doi: 10.1016/j.lfs.2012.05.021. Epub 2012 Jun 5.
Salvianolic acid B (SalB), one of the most abundant and bioactive compounds extracted from Salvia miltiorrhiza (Danshen), shows neuroprotective and anti-inflammatory activities in vivo and in vitro. This research was intended to investigate the antidepressant effect of SalB by forced swimming test (FST) and tail suspension test (TST).
SalB was extracted from S. miltiorrhiza roots and followed by HPLC analysis. Thirty five male C57BL/6 mice were divided into five groups: three SalB groups of different doses, one imipramine group, and one control group. The SalB groups received intraperitoneally (i.p.) 5mg/kg SalB, 10mg/kg SalB, and 20mg/kg SalB respectively. At the same time, the imipramine group received 20mg/kg imipramine, and the control group saline only. The behavioral tests including FST, TST and locomotor activity test were done after administration of drugs for consecutively three times, at 24, 1, and 0.5h before the tests.
SalB, from S. miltiorrhiza with purity of 95%, significantly reduced the immobility time in both the FST and TST tests (doses at 5, 10, 20mg/kg, i.p.), without changing locomotion in spontaneous motor activity.
This data suggests that besides neuroprotective and anti-inflammatory activities, SalB has promising therapeutic potential in treatment of depressive disorders.
丹酚酸 B（SalB）是从丹参中提取的最丰富和最具生物活性的化合物之一，具有体内和体外的神经保护和抗炎活性。本研究旨在通过强迫游泳试验（FST）和悬尾试验（TST）来研究 SalB 的抗抑郁作用。
从丹参根中提取 SalB，并进行 HPLC 分析。35 只雄性 C57BL/6 小鼠分为五组：三个不同剂量的 SalB 组、一个丙咪嗪组和一个对照组。SalB 组分别腹腔注射 5mg/kg SalB、10mg/kg SalB 和 20mg/kg SalB。同时，丙咪嗪组给予 20mg/kg 丙咪嗪，对照组给予生理盐水。给药后连续 3 次，在测试前 24、1 和 0.5 小时进行行为测试，包括 FST、TST 和自发运动活动测试。
纯度为 95%的来自丹参的 SalB 显著减少了 FST 和 TST 试验中的不动时间（5、10、20mg/kg，腹腔注射），而对自发运动活动没有影响。
这些数据表明，除了神经保护和抗炎活性外，SalB 在治疗抑郁症方面具有很大的治疗潜力。
