Mannan Abdul, Abir Ariful Basher, Rahman Rashidur
Department of Pharmacy, Stamford University Bangladesh, 51, Siddeswari Road, Dhaka, 1217, Bangladesh.
Department of Pharmacy, Faculty of Biological Science and Technology, Jessore University of Science and Technology, Jessore, 7408, Bangladesh.
BMC Complement Altern Med. 2015 Sep 25;15:337. doi: 10.1186/s12906-015-0866-2.
Bacopa monniera has been used as a cure for various ailments that include anxiety, epileptic disorders, dementia, blood purifier, cough and rheumatism, and some important local uses of the plant are in dermatitis, anemia, diabetes, promote fertility and prevent miscarriage for many years in Bangladesh. According to this background, the aim of the study was to evaluate the antidepressant-like effect of the methanolic extract of B. monniera (MEBM) in different behavioral models such as forced swimming test (FST), measurement of locomotor activity test (MLAT) and tail suspension test (TST) on mice after two weeks treatment.
Mice were divided into five groups (n = 5/group): control group (deionized water), standard group where Imipramine hydrochloride (30 mg/kg) was used as standard drug and three test groups where three doses of the methanolic extract of B. monniera (MEBM) (50, 100, and 200 mg/kg) was used for two weeks treatment. All the drug and test samples were administered via gavage through oral route. To assess the antidepressant-like effect of MEBM forced swimming test (FST), tail suspension test (TST) and measurement of locomotor activity test (MLAT) have been done in mice.
The results showed that a strong and dose-dependent antidepressant effects in different mice models. The main findings of the MEBM significantly reduced the duration of immobility times in the forced swimming test (p < 0.001). Likewise, the extract significantly decreased the immobility time in the tail suspension test (p < 0.001). Moreover, we employed an additional measurement of locomotor activity test to check the motor stimulating activity of the MEBM. The extract also significantly increased the locomotion, rearing and defecation effects in comparison to the control group (p < 0.001).
The present results clearly demonstrate that the methanolic extract of B. monniera possesses antidepressant-like activity in the animal behavioral models. The current study warrants further investigation into identification of the active compounds in herbal medicines, in particular extract of B. monniera with antidepressant-like effects.
多年来，在孟加拉国，假马齿苋一直被用于治疗各种疾病，包括焦虑、癫痫症、痴呆、血液净化、咳嗽和风湿病，该植物在当地的一些重要用途还包括治疗皮炎、贫血、糖尿病、促进生育和预防流产。基于此背景，本研究的目的是在连续两周的治疗后，通过强迫游泳试验（FST）、自发活动试验（MLAT）和悬尾试验（TST）等不同行为模型，评估假马齿苋甲醇提取物（MEBM）对小鼠的抗抑郁样作用。
将小鼠分为五组（每组n = 5）：对照组（去离子水）、以盐酸丙咪嗪（30 mg/kg）作为标准药物的标准组，以及三个试验组，分别给予三种剂量（50、100和200 mg/kg）的假马齿苋甲醇提取物（MEBM）进行为期两周的治疗。所有药物和试验样品均通过口服灌胃给药。为评估MEBM的抗抑郁样作用，对小鼠进行了强迫游泳试验（FST）、悬尾试验（TST）和自发活动试验（MLAT）。
结果表明，在不同小鼠模型中，MEBM具有显著且剂量依赖性的抗抑郁作用。MEBM的主要研究结果显示，在强迫游泳试验中，其显著缩短了不动时间（p < 0.001）。同样，在悬尾试验中，提取物也显著减少了不动时间（p < 0.001）。此外，我们还通过自发活动试验进行了额外测量，以检测MEBM的运动刺激活性。与对照组相比，提取物还显著增加了运动、直立和排便效果（p < 0.001）。
目前的结果清楚地表明，假马齿苋甲醇提取物在动物行为模型中具有抗抑郁样活性。当前的研究值得进一步探讨以确定草药中的活性成分，特别是具有抗抑郁样作用的确马齿苋提取物。
