Ohio State Biochemistry Program, Ohio State University, Columbus, OH 43210, USA.
FEBS Lett. 2012 Jul 30;586(16):2232-8. doi: 10.1016/j.febslet.2012.05.039. Epub 2012 Jun 7.
In archaea and eukaryotes aminoacyl-tRNA synthetases (aaRSs) associate in multi-synthetase complexes (MSCs), however the role of such MSCs in translation is unknown. MSC function was investigated in vivo in the archaeon Thermococcus kodakarensis, wherein six aaRSs were affinity co-purified together with several other factors involved in protein synthesis, suggesting that MSCs may interact directly with translating ribosomes. In support of this hypothesis, the aminoacyl-tRNA synthetase (aaRS) activities of the MSC were enriched in isolated T. kodakarensis polysome fractions. These data indicate that components of the archaeal protein synthesis machinery associate into macromolecular assemblies in vivo and provide the potential to increase translation efficiency by limiting substrate diffusion away from the ribosome, thus facilitating rapid recycling of tRNAs.
在古菌和真核生物中，氨酰-tRNA 合成酶（aaRS）与多合成酶复合物（MSC）相关联，然而，这种 MSC 在翻译中的作用尚不清楚。在古菌 Thermococcus kodakarensis 中，通过体内研究 MSC 的功能，发现六个 aaRS 与几个参与蛋白质合成的其他因子一起亲和共纯化，这表明 MSC 可能直接与正在翻译的核糖体相互作用。支持这一假说，MSC 的氨酰-tRNA 合成酶（aaRS）活性在分离的 T. kodakarensis 多核糖体部分中富集。这些数据表明，古菌蛋白质合成机制的成分在体内形成大分子组装体，并提供了通过限制底物从核糖体扩散来提高翻译效率的潜力，从而促进 tRNA 的快速循环。
