Plant Genome Research Unit Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Biochem Biophys Res Commun. 2012 Jun 29;423(2):417-23. doi: 10.1016/j.bbrc.2012.05.142. Epub 2012 Jun 5.
Thioredoxin (TRX) is a multi-functional redox protein. Genome-wide survey and expression profiles of different stresses were observed. Conserved amino acid residues and phylogeny construction using the OsTRX conserved domain sequence suggest that the TRX gene family can be classified broadly into six subfamilies in rice. We compared potential gene birth-and-death events in the OsTRX genes. The Ka/Ks ratio is a measure to explore the mechanism and 3 evolutionary stages of the OsTRX genes divergence after duplication. We used 270 TRX genes from monocots and eudicots for synteny analysis. Furthermore, we investigated expression profiles of this gene family under 5 biotic and 3 abiotic stresses. Several genes were differentially expressed with high levels of expression and exhibited subfunctionalization and neofunctionalization after the duplication event response to different stresses, which provides novel reference for the cloning of the most promising candidate genes from OsTRX gene family for further functional analysis.
硫氧还蛋白（TRX）是一种多功能的氧化还原蛋白。观察了全基因组调查和不同应激的表达谱。使用 OsTRX 保守结构域序列构建保守氨基酸残基和系统发育表明，TRX 基因家族可广泛分为水稻中的六个亚家族。我们比较了 OsTRX 基因中潜在的基因诞生和消亡事件。Ka/Ks 比值是探索 OsTRX 基因复制后分化机制和 3 个进化阶段的一种衡量标准。我们使用来自单子叶植物和双子叶植物的 270 个 TRX 基因进行基因同线性分析。此外，我们研究了该基因家族在 5 种生物和 3 种非生物胁迫下的表达谱。一些基因在复制事件后表现出高水平的差异表达，并对不同胁迫表现出亚功能化和新功能化，这为从 OsTRX 基因家族克隆最有前途的候选基因进行进一步功能分析提供了新的参考。
