Sainz María Martha, Filippi Carla Valeria, Eastman Guillermo, Sotelo-Silveira José, Borsani Omar, Sotelo-Silveira Mariana
Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Avenida Garzón 780, Montevideo 12900, Uruguay.
Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, MEC, Av. Italia 3318, Montevideo 11600, Uruguay.
Antioxidants (Basel). 2022 Aug 21;11(8):1622. doi: 10.3390/antiox11081622.
Soybean ( (L.) Merr.) establishes symbiosis with rhizobacteria, developing the symbiotic nodule, where the biological nitrogen fixation (BNF) occurs. The redox control is key for guaranteeing the establishment and correct function of the BNF process. Plants have many antioxidative systems involved in ROS homeostasis and signaling, among them a network of thio- and glutaredoxins. Our group is particularly interested in studying the differential response of nodulated soybean plants to water-deficit stress. To shed light on this phenomenon, we set up an RNA-seq experiment (for total and polysome-associated mRNAs) with soybean roots comprising combined treatments including the hydric and the nodulation condition. Moreover, we performed the initial identification and description of the complete repertoire of thioredoxins (Trx) and glutaredoxins (Grx) in soybean. We found that water deficit altered the expression of a greater number of differentially expressed genes (DEGs) than the condition of plant nodulation. Among them, we identified 12 thioredoxin (Trx) and 12 glutaredoxin (Grx) DEGs, which represented a significant fraction of the detected GmTrx and GmGrx in our RNA-seq data. Moreover, we identified an enriched network in which a GmTrx and a GmGrx interacted with each other and associated through several types of interactions with nitrogen metabolism enzymes.
大豆（(L.) Merr.）与根际细菌建立共生关系，形成共生根瘤，生物固氮（BNF）在此发生。氧化还原控制是保证BNF过程建立和正常功能的关键。植物有许多参与活性氧（ROS）稳态和信号传导的抗氧化系统，其中包括硫氧还蛋白和谷氧还蛋白网络。我们团队特别关注研究结瘤大豆植株对水分亏缺胁迫的差异响应。为了阐明这一现象，我们对大豆根进行了RNA测序实验（针对总mRNA和多聚核糖体相关mRNA），实验采用了包括水分状况和结瘤状况的联合处理。此外，我们对大豆中硫氧还蛋白（Trx）和谷氧还蛋白（Grx）的完整库进行了初步鉴定和描述。我们发现，水分亏缺比植株结瘤状况改变了更多差异表达基因（DEG）的表达。其中，我们鉴定出12个硫氧还蛋白（Trx）差异表达基因和12个谷氧还蛋白（Grx）差异表达基因，它们在我们的RNA测序数据中占已检测到的大豆硫氧还蛋白（GmTrx）和谷氧还蛋白（GmGrx）的很大一部分。此外，我们鉴定出一个富集网络，其中一个大豆硫氧还蛋白（GmTrx）和一个大豆谷氧还蛋白（GmGrx）相互作用，并通过几种类型的相互作用与氮代谢酶相关联。
