Biomolecular Science, University of Science and Technology, Daejon 305-806, Republic of Korea.
Eur J Pharmacol. 2012 Aug 15;689(1-3):38-44. doi: 10.1016/j.ejphar.2012.05.050. Epub 2012 Jun 7.
Chronic exposure of human skin to solar ultraviolet (UV) radiation causes photoaging. Naturally occurring phytochemicals are known to have anti-photoaging effects. The present study examined the effect of mangiferin isolated from Anemarrhena asphodeloides on wrinkle formation, skin thickness, and changes in collagen fibers in hairless mice. The in vitro effects and possible mechanism of mangiferin on UVB irradiation were determined in human keratinocyte (HEKa) cells. In vitro results showed that mangiferin reduced UVB-induced matrix metalloproteinase (MMP)-9 protein expression and enzyme activity and subsequent attenuation of UVB-induced phosphorylation of mitogen-activated protein kinase kinase1 (MEK) and extracellular signal-regulated kinase (ERK). In the in vivo studies, mangiferin inhibited UVB-induced mean length and mean depth of skin wrinkle based on skin replica, epidermal thickening, and damage to collagen fiber. Taken together, these results indicate that mangiferin exerts anti-photoaging activity in UVB-irradiated hairless mice by regulating MMP-9 expression through inhibition of MEK and ERK.
人体皮肤长期暴露在太阳紫外线（UV）辐射下会导致光老化。已知天然存在的植物化学物质具有抗光老化作用。本研究探讨了从知母中分离得到的芒果苷对无毛小鼠皱纹形成、皮肤厚度和胶原纤维变化的影响。在体外，研究了芒果苷对 UVB 照射的影响及其可能的机制，在人角质形成细胞（HEKa）中进行了研究。体外结果表明，芒果苷减少了 UVB 诱导的基质金属蛋白酶（MMP）-9 蛋白表达和酶活性，以及随后 UVB 诱导的丝裂原活化蛋白激酶激酶 1（MEK）和细胞外信号调节激酶（ERK）的磷酸化。在体内研究中，芒果苷抑制了 UVB 诱导的皮肤皱纹平均长度和平均深度，基于皮肤复制品，还抑制了表皮增厚和胶原纤维损伤。综上所述，这些结果表明，芒果苷通过抑制 MEK 和 ERK 调节 MMP-9 的表达，对 UVB 照射的无毛小鼠发挥抗光老化作用。
