猪肾D-天冬氨酸氧化酶的结晶及初步晶体学分析
Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney.
作者信息
Senda Miki, Yamamoto Atsushi, Tanaka Hiroyuki, Ishida Tetsuo, Horiike Kihachiro, Senda Toshiya
机构信息
Structure Guided Drug Development Project, JBIC Research Institute, Japan Biological Informatics Consortium, 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):644-6. doi: 10.1107/S1744309112013243. Epub 2012 May 22.
Abstract
D-Aspartate oxidase (DDO) from porcine kidney was crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as a precipitant. The crystal belonged to space group P2(1), with unit-cell parameters a = 79.38, b = 144.0, c = 80.46 Å, β = 101.1°, and diffracted to 1.80 Å resolution. Molecular-replacement trials using the structure of human D-amino-acid oxidase, which is 42% identical in sequence to DDO, as a search model provided a satisfactory solution.
摘要
使用聚乙二醇8000作为沉淀剂，通过坐滴气相扩散法使来自猪肾的D-天冬氨酸氧化酶（DDO）结晶。该晶体属于空间群P2(1)，晶胞参数为a = 79.38、b = 144.0、c = 80.46 Å，β = 101.1°，衍射分辨率达到1.80 Å。以与DDO序列有42%同一性的人D-氨基酸氧化酶的结构作为搜索模型进行分子置换试验，得到了令人满意的结果。
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