Ichiyanagi Atsushi, Hirokawa Kozo, Gomi Keiko, Nakatsu Toru, Kato Hiroaki, Kajiyama Naoki
Research and Development Division, Kikkoman Corporation, 399 Noda, Noda-shi, Chiba 278-0037, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):130-3. doi: 10.1107/S1744309112051445. Epub 2013 Jan 30.
Fructosyl peptide oxidase (FPOX) catalyses the oxidation of α-glycated dipeptides such as N(α)-(1-deoxy-D-fructos-1-yl)-L-valyl-L-histidine (Fru-ValHis) and is used in the diagnosis of diabetes mellitus. Here, two thermostable mutants of FPOX, CFP-T7 and EFP-T5M, were crystallized by the sitting-drop vapour-diffusion method. The crystal of CFP-T7 belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 110.09, c = 220.48 Å, and that of EFP-T5M belonged to the monoclinic space group P2(1), with unit-cell parameters a = 43.00, b = 230.05, c = 47.27 Å, β = 116.99°. The crystals of CFP-T7 and EFP-T5M diffracted to 1.8 and 1.6 Å resolution, respectively.
果糖基肽氧化酶（FPOX）催化α-糖化二肽的氧化，如N（α）-（1-脱氧-D-果糖-1-基）-L-缬氨酰-L-组氨酸（Fru-ValHis），并用于糖尿病的诊断。在此，通过坐滴气相扩散法使FPOX的两个热稳定突变体CFP-T7和EFP-T5M结晶。CFP-T7的晶体属于四方晶系空间群P4(1)2(1)2，晶胞参数a = b = 110.09，c = 220.48 Å，EFP-T5M的晶体属于单斜晶系空间群P2(1)，晶胞参数a = 43.00，b = 230.05，c = 47.27 Å，β = 116.99°。CFP-T7和EFP-T5M的晶体分别衍射至1.8 Å和1.6 Å的分辨率。
