State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Biol Trace Elem Res. 2012 Dec;150(1-3):264-71. doi: 10.1007/s12011-012-9462-1. Epub 2012 Jun 10.
Lead causes a broad range of adverse effects in humans and animals. The objective was to evaluate the potency of lactobacilli to bind lead in vitro and the protective effects of a selected Lactobacillus plantarum CCFM8661 against lead-induced toxicity in mice. Nine strains of bacteria were used to investigate their binding abilities of lead in vitro, and L. plantarum CCFM8661 was selected for animal experiments because of its excellent lead binding capacity. Both living and dead L. plantarum CCFM8661 were used to treat 90 male Kunming mice during or after the exposure to 1 g/L lead acetate in drinking water. The results showed oral administration of both living and dead L. plantarum CCFM8661 offered a significant protective effect against lead toxicity by recovering blood δ-aminolevulinic acid dehydratase activity, decreasing the lead levels in blood and tissues, and preventing alterations in the levels of glutathione, glutathione peroxidase, malondialdehyde, superoxide dismutase, and reactive oxygen species caused by lead exposure. Moreover, L. plantarum CCFM8661 was more effective when administered consistently during the entire lead exposure, not after the exposure. Our results suggest that L. plantarum CCFM8661 has the potency to provide a dietary strategy against lead toxicity.
铅在人类和动物体内会引起广泛的不良反应。本研究旨在评估乳酸菌体外结合铅的能力，并研究一株植物乳杆菌 CCFM8661 对铅诱导的小鼠毒性的保护作用。使用了 9 株细菌来研究它们的体外结合铅的能力，由于其优异的铅结合能力，选择植物乳杆菌 CCFM8661 用于动物实验。在饮用水中暴露于 1g/L 醋酸铅后，用活菌和死菌的植物乳杆菌 CCFM8661 治疗 90 只雄性昆明小鼠。结果表明，活菌和死菌的植物乳杆菌 CCFM8661 口服给药均能通过恢复血液 δ-氨基乙酰丙酸脱水酶活性、降低血液和组织中的铅含量、防止因铅暴露引起的谷胱甘肽、谷胱甘肽过氧化物酶、丙二醛、超氧化物歧化酶和活性氧水平的改变，对铅毒性提供显著的保护作用。此外，活菌的植物乳杆菌 CCFM8661 在整个铅暴露期间持续给药比暴露后给药更有效。我们的研究结果表明，植物乳杆菌 CCFM8661 具有提供针对铅毒性的饮食策略的潜力。
