School of Agriculture, Tokai University, Kumamoto, Japan.
Methods Mol Biol. 2024;2851:201-212. doi: 10.1007/978-1-0716-4096-8_18.
The gradual accumulation of heavy metals can have detrimental effects on health. Lactic acid bacteria (LAB) are common microbes used as probiotics; various LAB strains are consumed in food products, especially in fermented foods. Many studies have suggested that LAB with high affinity to harmful heavy metals can be used as efficient detoxification tools. Accordingly, it is important to test the biosorption of various heavy metals, e.g., cadmium, lead, arsenic, and mercury, by LAB. Here, I describe protocols to quantify the binding ability of LAB and to identify their heavy metal-binding proteins.
重金属的逐渐积累可能对健康产生有害影响。乳酸菌 (LAB) 是常用的益生菌微生物；各种 LAB 菌株被用于食品产品，尤其是发酵食品。许多研究表明，对有害重金属具有高亲和力的 LAB 可以用作有效的解毒工具。因此，测试 LAB 对各种重金属（例如镉、铅、砷和汞）的生物吸附能力非常重要。在这里，我描述了定量测定 LAB 结合能力并鉴定其重金属结合蛋白的方案。
