通过共价交联稳定功能化小半导体聚合物点及其在特定细胞成像中的应用。
Stable functionalization of small semiconducting polymer dots via covalent cross-linking and their application for specific cellular imaging.
机构信息
Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
出版信息
Adv Mater. 2012 Jul 10;24(26):3498-504. doi: 10.1002/adma.201201245. Epub 2012 Jun 11.
Abstract
A facile cross-linking strategy covalently links functional molecules to semiconducting polymer dots (Pdots) while simultaneously providing functional groups for biomolecular conjugation. In addition to greatly enhanced stability, the formed Pdots are small (<10 nm), which can be difficult to achieve with current methods but is highly desirable for most biological applications. These characteristics are significant for improving labeling efficiency and sensitivity in cellular assays that employ Pdots.
摘要
一种简单的交联策略将功能分子共价连接到半导体聚合物点（Pdots）上，同时为生物分子缀合提供了功能基团。除了大大提高稳定性外，形成的 Pdots 很小（<10nm），这很难用目前的方法实现，但对于大多数生物应用来说是非常理想的。这些特性对于提高细胞分析中使用 Pdots 的标记效率和灵敏度具有重要意义。
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