应用 pheB 作为地芽孢杆菌属的报告基因，实现了定性的菌落筛选和启动子强度的定量分析。
Application of pheB as a reporter gene for Geobacillus spp., enabling qualitative colony screening and quantitative analysis of promoter strength.
机构信息
Department of Life Sciences, Imperial College London, London, United Kingdom.
出版信息
Appl Environ Microbiol. 2012 Aug;78(16):5945-7. doi: 10.1128/AEM.07944-11. Epub 2012 Jun 8.
Abstract
The pheB gene from Geobacillus stearothermophilus DSM6285 has been exploited as a reporter gene for Geobacillus spp. The gene product, catechol 2,3-dioxygenase (C23O), catalyzes the formation of 2-hydroxymuconic semialdehyde, which can be readily assayed. The reporter was used to examine expression from the ldh promoter associated with fermentative metabolism.
摘要
来自嗜热脂肪地芽孢杆菌 DSM6285 的 pheB 基因被用作地芽孢杆菌属的报告基因。该基因产物儿茶酚 2,3-双加氧酶 (C23O) 催化 2-羟粘康酸半醛的形成，可方便地进行测定。该报告基因用于检测与发酵代谢相关的 ldh 启动子的表达。
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