School of Biology, Newcastle University, Newcastle upon Tyne, United Kingdom.
PLoS One. 2012;7(6):e38515. doi: 10.1371/journal.pone.0038515. Epub 2012 Jun 7.
To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.
为了评估一种快速且经济有效的监测压载水中细菌的方法，使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对几种海洋细菌分离株进行了表征。由于国际海事组织（IMO）的法规涉及到通过压载水意外运输致病性细菌，因此重点检测了弧菌、肠球菌和大肠菌群。从北海采集的海水样本在钢压载舱中进行培养，并研究了假单胞菌中潜在有害物种的存在。使用 MALDI-TOF MS 在属水平上对 36 个分离株进行鉴定，得到的结果与 16S rRNA 基因测序获得的结果相似。除机会性病原体铜绿假单胞菌外，通过 16S rRNA 基因分析或 MALDI-TOF MS 均未检测到致病物种。此外，还开发并使用了一种内部软件，该软件计算了质谱原始数据的相关系数值（CCV）及其变化，首次允许快速有效地鉴定压载水中的海洋细菌。
