CETESB - Companhia Ambiental do Estado de São Paulo, Department of Environmental Analysis, São Paulo - SP, Brazil.
UNIFESP - Universidade Federal de São Paulo, Department of Medicine, ALERTA Laboratory, São Paulo - SP, Brazil.
Mar Pollut Bull. 2017 May 15;118(1-2):376-381. doi: 10.1016/j.marpolbul.2017.03.025. Epub 2017 Mar 17.
MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMérieux's API® 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods. The 16S rRNA gene sequencing of isolates with discrepant results showed that MALDI-TOF and API® correctly identified 74% and 11% of these isolates, respectively. This discrepancy probably relies on the bias of the API® has to identify clinical isolates. MALDI-TOF proved to be a feasible approach for identifying Enterococcus from environmental matrices increasing the rapidness and accuracy of results.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）生物分型已被证明是一种可靠的方法，可基于核糖体蛋白质量指纹图谱分析来鉴定细菌种属。我们评估了该方法在鉴定从巴西海滩海洋休闲水中分离出的肠球菌种属的有用性。通过生物梅里埃公司的 API® 20 Strep 和 MALDI-TOF 系统，将 127 株肠球菌 spp. 分离物鉴定到种属水平。生化试验鉴定了 117/127 株（92%），而 MALDI 鉴定了 100%的分离物，两种方法的一致性为 63%。对结果不一致的分离物进行 16S rRNA 基因测序显示，MALDI-TOF 和 API®分别正确鉴定了这些分离物中的 74%和 11%。这种差异可能依赖于 API®在鉴定临床分离物时的偏向性。MALDI-TOF 被证明是一种从环境基质中鉴定肠球菌的可行方法，提高了结果的快速性和准确性。
