Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Free Radic Res. 2012 Oct;46(10):1230-7. doi: 10.3109/10715762.2012.701291. Epub 2012 Jul 4.
The significance of 5-lipoxygenase and myeloperoxidase activities has not been extensively studied among young male smokers. Leukotriene B(4), 20-hydroxy-leukotriene B(4), 20-carboxy-leukotriene B(4) and 3-chlorotyrosine were measured in plasma and urinary samples of young male smokers at 8 hours following cigarette abstinence and an hour after cigarette smoking. Leukotriene B(4) and 3-chlorotyrosine were determined in neutrophils isolated from these individuals. The levels of these markers were compared with those of age-matched controls. In vitro studies were performed to evaluate the production of leukotriene B(4) and 3-chlorotyrosine from human neutrophils following exposure to nicotine and cotinine. Thirty male smokers (mean age, 27.4 years) and 28 male non-smokers (mean age, 28.7 years) were studied. Plasma levels of leukotriene B(4), 20-carboxy-leukotriene B(4) and 3-chlorotyrosine were higher in smokers than in non-smokers; leukotriene B(4) and 20-carboxy-leukotriene B(4) levels increased further an hour after cigarette smoking. Peripheral neutrophils isolated from smokers showed greater expressions of myeloperoxidase and 5-lipoxygenase activities compared with non-smokers, while plasma leukotriene B(4) and 3-chlorotyrosine were correlated significantly with high-sensitivity C-reactive protein and plasma nicotine concentrations. Exposure of human neutrophils to nicotine and cotinine resulted in a higher production of leukotriene B(4) and 3-chlorotyrosine. To conclude, leukotriene B(4) and 3-chlorotyrosine levels are increased in young male cigarette smokers. These results suggest that cigarette smoking aggravates neutrophil-mediated inflammation by modulating the activities of myeloperoxidase and 5-lipoxygenase pathways.
5-脂氧合酶和髓过氧化物酶活性在年轻男性吸烟者中的意义尚未得到广泛研究。在戒烟 8 小时和吸烟 1 小时后，测量了年轻男性吸烟者的血浆和尿液样本中的白三烯 B（4）、20-羟基白三烯 B（4）、20-羧基白三烯 B（4）和 3-氯酪氨酸。从这些个体分离的中性粒细胞中测定了白三烯 B（4）和 3-氯酪氨酸。将这些标志物的水平与年龄匹配的对照组进行比较。进行了体外研究，以评估暴露于尼古丁和可替宁后人类中性粒细胞产生白三烯 B（4）和 3-氯酪氨酸的情况。研究了 30 名男性吸烟者（平均年龄 27.4 岁）和 28 名男性非吸烟者（平均年龄 28.7 岁）。吸烟者的血浆白三烯 B（4）、20-羧基白三烯 B（4）和 3-氯酪氨酸水平高于非吸烟者；吸烟后 1 小时，白三烯 B（4）和 20-羧基白三烯 B（4）水平进一步升高。与非吸烟者相比，从吸烟者中分离出的外周中性粒细胞显示出更高的髓过氧化物酶和 5-脂氧合酶活性表达，而血浆白三烯 B（4）和 3-氯酪氨酸与高敏 C 反应蛋白和血浆尼古丁浓度显著相关。暴露于尼古丁和可替宁的人类中性粒细胞导致白三烯 B（4）和 3-氯酪氨酸的产量增加。总之，年轻男性吸烟者中白三烯 B（4）和 3-氯酪氨酸水平升高。这些结果表明，吸烟通过调节髓过氧化物酶和 5-脂氧合酶途径的活性来加重中性粒细胞介导的炎症。
