Departmentof Biomedical Engineering, University of Sussex, Brighton, UK.
IEEE Trans Biomed Eng. 2012 Aug;59(8):2321-9. doi: 10.1109/TBME.2012.2202904. Epub 2012 Jun 6.
The bioimpedance spectroscopy (BIS) technique is potentially a useful tool to differentiate malignancy based on the variation of electrical properties presented by different tissues and cells. The different tissues and cells present variant electrical resistance and reactance when excited at different frequencies. The main purpose of this area of research is to use impedance measurements over a low-frequency bandwidth ranging from 1 kHz to 3 MHz to 1) differentiate the pathological stages of cancer cells under laboratory conditions and 2) permit the extraction of electrical parameters related to cellular information for further analysis. This provides evidence to form the basis of bioimpedance measurement at the cellular level and aids the potential future development of rapid diagnostics from biopsy materials. Three cell lines, representing normal breast epithelia and different pathological stages of breast cancer, have been measured using a standard impedance analyzer driving a four-electrode chamber filled with different cell suspensions. We identify the specific BIS profile for each cell type and determine whether these can be differentiated. In addition, the electrical parameters, e.g., the intracellular conductivity, membrane capacitance/capacity, characteristic frequency, are extracted by the use of equivalent circuit models and physical models to provide details of the cell electric signatures for further analysis of cancer cell pathology.
生物阻抗谱（BIS）技术是一种有潜力的工具，可以根据不同组织和细胞呈现的电特性变化来区分恶性肿瘤。不同的组织和细胞在不同频率下受到激励时会呈现出不同的电阻和电抗。该研究领域的主要目的是使用低频带宽（1 kHz 至 3 MHz）内的阻抗测量值：1）在实验室条件下区分癌细胞的病理阶段，2）提取与细胞信息相关的电参数以进行进一步分析。这为在细胞水平进行生物阻抗测量提供了依据，并为从活检材料中快速诊断的潜在未来发展提供了帮助。使用标准阻抗分析仪驱动填充有不同细胞悬浮液的四电极室，对三种细胞系（代表正常乳腺上皮和不同阶段的乳腺癌）进行了测量。我们确定了每种细胞类型的特定 BIS 图谱，并确定是否可以对其进行区分。此外，还通过使用等效电路模型和物理模型提取电参数，如细胞内电导率、细胞膜电容/容量、特征频率，以提供细胞电签名的详细信息，用于进一步分析癌细胞病理学。
