Nakata Natsumi, Ishibashi Yuko, Miyata Shogo
Department of Mechanical Engineering, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
Micromachines (Basel). 2022 May 27;13(6):837. doi: 10.3390/mi13060837.
The quantitative and functional analyses of cells are important for cell-based therapies. In this study, to establish the quantitative cell analysis method, we propose an impedance measurement method supported by dielectrophoretic cell accumulation. An impedance measurement and dielectrophoresis device was constructed using opposing comb-shaped electrodes. Using dielectrophoresis, cells were accumulated to form chain-like aggregates on the electrodes to improve the measurement sensitivity of the electrical impedance device. To validate the proposed method, the electrical impedance and capacitance of primary and de-differentiated chondrocytes were measured. As a result, the impedance of the chondrocytes decreased with an increase in the passage number, whereas the capacitance increased. Therefore, the impedance measurement method proposed in this study has the potential to identify chondrocyte phenotypes.
细胞的定量和功能分析对于基于细胞的治疗至关重要。在本研究中，为建立定量细胞分析方法，我们提出一种由介电泳细胞聚集支持的阻抗测量方法。使用相对的梳状电极构建了一种阻抗测量和介电泳装置。利用介电泳，细胞在电极上聚集形成链状聚集体，以提高电阻抗装置的测量灵敏度。为验证所提出的方法，测量了原代和去分化软骨细胞的电阻抗和电容。结果，软骨细胞的阻抗随传代次数增加而降低，而电容增加。因此，本研究提出的阻抗测量方法有潜力识别软骨细胞表型。
