Heymans Raymond, A Matser Amy, Bruisten Sylvia M, Heijman Titia, Geskus Ronald B, Speksnijder Adrianus G C L, Davidovich Udi, de Vries Henry J C, Coutinho Roel A, Schim van der Loeff Maarten F
Public Health Service (GGD) of Amsterdam, Cluster of Infectious Diseases, Department of Research, Postbox 2200, 1000 CE Amsterdam, The Netherlands.
J Infect Dis. 2012 Aug 15;206(4):596-605. doi: 10.1093/infdis/jis399. Epub 2012 Jun 12.
Molecular typing was used to elucidate Neisseria gonorrhoeae transmission networks among men who have sex with men (MSM) in Amsterdam, the Netherlands. We determined whether clusters of patients infected with specific N. gonorrhoeae genotypes were related to various epidemiological characteristics.
MSM (age ≥18 years) visiting the sexually transmitted infections (STI) clinic between July 2008 and August 2009 were eligible. After STI screening, participants completed a behavioral questionnaire concerning the previous 6 months. N. gonorrhoeae cultures were genotyped using multiple-locus variable-number tandem repeat analysis typing.
We obtained 278 N. gonorrhoeae-positive isolates from 240 MSM. Five large clusters (≥10 isolates), a unique sixth cluster (n = 9), and 8 smaller clusters (5-9 isolates) were identified. Prevalence of human immunodeficiency virus differed between clusters I and VI (P = .003), ranging from 27.8% to 100%. Receptive unprotected anal intercourse was frequently reported by MSM (51.8%) but did not differ significantly among clusters. Significant differences were identified concerning the participant's history of syphilis (P = .030), having met partners at a popular sex venue in Amsterdam (P = .048), and meeting partners outside Amsterdam (P = .036).
Distinct N. gonorrhoeae transmission networks were present in a mixed high-risk MSM population; concordance between clusters and epidemiological characteristics was present but not marked.
采用分子分型方法阐明荷兰阿姆斯特丹男男性行为者（MSM）中淋病奈瑟菌的传播网络。我们确定感染特定淋病奈瑟菌基因型的患者群是否与各种流行病学特征相关。
符合条件的为2008年7月至2009年8月间到性传播感染（STI）门诊就诊的年龄≥18岁的MSM。在进行性传播感染筛查后，参与者完成一份关于前6个月行为的问卷。采用多位点可变数目串联重复序列分析分型对淋病奈瑟菌培养物进行基因分型。
我们从240名MSM中获得了278株淋病奈瑟菌阳性分离株。确定了5个大簇（≥10株分离株）、一个独特的第六簇（n = 9）和8个较小的簇（5 - 9株分离株）。I簇和VI簇之间的人类免疫缺陷病毒患病率不同（P = .003），范围从27.8%至100%。MSM经常报告接受无保护肛交（51.8%），但各簇之间无显著差异。在梅毒病史（P = .030）、在阿姆斯特丹一个热门性场所结识性伴（P = .048）以及在阿姆斯特丹以外结识性伴（P = .036）方面发现了显著差异。
在一个混合的高风险MSM人群中存在不同的淋病奈瑟菌传播网络；各簇与流行病学特征之间存在一致性，但不明显。
