Ozcan B, Ozyilmaz G, Cihan A, Cokmus C, Caliskan M
Mustafa Kemal University, Biology Department, Faculty of Sciences and Letters, Turkey.
Mikrobiologiia. 2012 Mar-Apr;81(2):205-13.
Five isolates designated as B45, D83A, A206A, A85 and E49 and found to possess a activity were taxonomically classified on the basis of their phylogenetic, phenotypic and chemotaxonomic characteristics. The isolates were determined to be Gram-negative, catalase and oxidase positive, hydrolyzing Tween 80 and 60 but not starch, need 3.5-4 M NaCl for optimal growth and lack of anaerobic growth with arginine or DMSO. All isolates had the highest lipolytic activity at pH 8.5. Lipase and esterase activities increased with salt concentration up to 3-4.5 M NaCl, and decreased at 5 M NaCl. Esterase and lipase showed their maximal activities at 50-55 degrees C and 60-65 degrees C, respectively. The phylogenetic tree constructed by the neighbor-joining method indicated that the strain B45 and A85 were closely related to the members of genera Halovivax and Natrinema, respectively. The closest relative of the strain A206A and D83A were found to be Haloterrigena saccharevitans. The strain E49 displayed a more distant relationship to known strains.
五个被命名为B45、D83A、A206A、A85和E49的分离株被发现具有一种活性，并根据它们的系统发育、表型和化学分类特征进行了分类学归类。这些分离株被确定为革兰氏阴性，过氧化氢酶和氧化酶阳性，能水解吐温80和吐温60但不能水解淀粉，最佳生长需要3.5 - 4 M氯化钠，在以精氨酸或二甲基亚砜为底物时不能进行厌氧生长。所有分离株在pH 8.5时具有最高的脂肪分解活性。脂肪酶和酯酶活性随盐浓度增加至3 - 4.5 M氯化钠而增加，在5 M氯化钠时降低。酯酶和脂肪酶分别在50 - 55℃和60 - 65℃时表现出最大活性。通过邻接法构建的系统发育树表明，菌株B45和A85分别与嗜盐栖菌属和嗜盐放线菌属的成员密切相关。发现菌株A206A和D83A的最接近亲缘种是嗜盐糖生盐地芽孢杆菌。菌株E49与已知菌株的关系更为疏远。
