Gutiérrez M C, Castillo A M, Kamekura M, Ventosa A
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Sevilla, Spain.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2880-4. doi: 10.1099/ijs.0.2008/001602-0.
A novel extremely halophilic strain, designated XH-65(T), isolated from the salt lake Xilinhot in Inner Mongolia, PR China, was subjected to a polyphasic taxonomic characterization. Strain XH-65(T) is neutrophilic, non-motile and requires at least 2.5 M NaCl for growth, with an optimum at 3.4 M NaCl, and grows at pH 6.0-9.0, with optimum growth at pH 7.5. Strain XH-65(T) grows at 25-50 degrees C, with optimal growth at 37 degrees C. Magnesium is not required for growth. On the basis of 16S rRNA gene sequence analysis, strain XH-65(T) was shown to belong to the genus Haloterrigena and was related to Haloterrigena turkmenica VKM B-1734(T) (98.1 % sequence similarity), Haloterrigena saccharevitans AB14(T) (96.9 %), Haloterrigena thermotolerans PR5(T) (96.3 %), Haloterrigena limicola AX-7(T) (95.8 %) and Haloterrigena hispanica FP1(T) (95.7 %). DNA-DNA hybridization revealed 37 % relatedness between strain XH-65(T) and Htg. turkmenica VKM B-1734(T). The polar lipid composition revealed the presence of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1-->2)-glucose glycerol diether (S(2)-DGD). The results of the DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain XH-65(T) from the six Haloterrigena species with validly published names. Therefore, strain XH-65(T) represents a novel species, for which the name Haloterrigena salina sp. nov. is proposed, with the type strain XH-65(T) (=CGMCC 1.6203(T) =JCM 13891(T)).
从中国内蒙古锡林浩特盐湖分离出的一株新型极端嗜盐菌株，命名为XH-65(T)，对其进行了多相分类学表征。菌株XH-65(T)为嗜中性、不运动，生长需要至少2.5 M NaCl，最适NaCl浓度为3.4 M，在pH 6.0 - 9.0范围内生长，最适生长pH为7.5。菌株XH-65(T)在25 - 50℃生长，最适生长温度为37℃。生长不需要镁。基于16S rRNA基因序列分析，菌株XH-65(T)被证明属于嗜盐栖地杆菌属，与土库曼嗜盐栖地杆菌VKM B-1734(T)（序列相似性98.1%）、蔗糖嗜盐栖地杆菌AB14(T)（96.9%）、耐热嗜盐栖地杆菌PR5(T)（96.3%）、盐沼嗜盐栖地杆菌AX-7(T)（95.8%）和西班牙嗜盐栖地杆菌FP1(T)（95.7%）相关。DNA-DNA杂交显示菌株XH-65(T)与土库曼嗜盐栖地杆菌VKM B-1734(T)之间的相关性为37%。极性脂质组成显示存在磷脂酰甘油、磷脂酰甘油磷酸甲酯和甘露糖-2,6-二硫酸酯(1→2)-葡萄糖甘油二醚（S(2)-DGD）。DNA-DNA杂交以及生理生化试验结果使得菌株XH-65(T)能够与已有效发表名称的六个嗜盐栖地杆菌属物种在基因型和表型上区分开来。因此，菌株XH-65(T)代表一个新物种，提议将其命名为盐嗜盐栖地杆菌（Haloterrigena salina sp. nov.），模式菌株为XH-65(T)（=CGMCC 1.6203(T)=JCM 13891(T)）。
