Ghasemi Y, Rasoul-Amini S, Kazemi A, Zarrinic G, Morowvat M H, Kargar M
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran.
Mikrobiologiia. 2011 Jul-Aug;80(4):477-81.
Lipases are an important class of enzymes which catalyze the hydrolysis of long chain triglycerides and constitute the most prominent group ofbiocatalysts for biotechnological applications. There are a number of lipases, produced by some halophilic microorganisms. In this study, some lipase producing bacteria from Maharlu salt lake located in south of Iran were isolated. All isolates were screened for true lipase activity on plates containing olive oil. The lipase activity was measured using titrimetric methods. Among thirty three isolates, thirteen strains demonstrating orange zone around colonies under UV light, were selected for identification using the molecular methods and some morphological characteristics. The bacterium Bacillus vallismortis BCCS 007 with 3.41 +/- 0.14 U/mL lipase activity was selected as the highest lipase producing isolate. This is the first report of isolation and molecular identification of lipase producing bacteria from Maharlu lake.
脂肪酶是一类重要的酶，可催化长链甘油三酯的水解，是生物技术应用中最突出的生物催化剂类别。一些嗜盐微生物能产生多种脂肪酶。在本研究中，从伊朗南部的马哈尔卢盐湖分离出了一些产脂肪酶的细菌。在含有橄榄油的平板上对所有分离株进行了真脂肪酶活性筛选。采用滴定法测定脂肪酶活性。在33株分离株中，选择了13株在紫外光下菌落周围呈现橙色区域的菌株，利用分子方法和一些形态学特征进行鉴定。脂肪酶活性为3.41±0.14 U/mL的瓦利斯芽孢杆菌BCCS 007被选为产脂肪酶最高的分离株。这是首次从马哈尔卢湖分离出产脂肪酶细菌并进行分子鉴定的报告。
