Li Yanpeng, Geng Lu, Chen Jing, Huang Ying, Qiao Jing, Ren Guangxi, Liu Chunsheng
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, 100102 China.
3 Biotech. 2018 Feb;8(2):79. doi: 10.1007/s13205-018-1085-6. Epub 2018 Jan 15.
Previous studies found that cytokinins (CTKs) are able to significantly promote glycyrrhizic acid (GA) accumulation. This study analyzed the correlation between the contents of GA and CTKs in 98 one-year-old liquorice plants by the combination of high-performance liquid chromatography and enzyme-linked immunosorbent assay. The full length of the liquorice IPT gene was cloned and the Single-nucleotide polymorphisms (SNPs) of the IPT gene in the samples were obtained. The molecular marker of high GA content was found by performing correlation analysis on the SNPs of the IPT gene. Results indicated that the contents of GA and CTKs were positively correlated. The full-length sequence of the IPT gene was 1002 bps, and five SNPs were found in the 98 liquorice samples. One of five SNPs was 348 bps, exhibiting missense mutation and the rest displaying synonymous mutations. In the five SNPs, 348 bps was primarily associated SNP, whereas 821 and 848 bps were auxiliary associated SNP. The findings of this study provided insight into the molecular mechanism of GA genetic variation, serving as basis for improving the GA content in cultivated liquorice and the good varieties breeding.
先前的研究发现，细胞分裂素（CTKs）能够显著促进甘草酸（GA）的积累。本研究通过高效液相色谱法和酶联免疫吸附测定法相结合，分析了98株一年生甘草植株中GA和CTKs含量之间的相关性。克隆了甘草IPT基因的全长，并获得了样品中IPT基因的单核苷酸多态性（SNPs）。通过对IPT基因的SNPs进行相关性分析，发现了高GA含量的分子标记。结果表明，GA和CTKs的含量呈正相关。IPT基因的全长序列为1002 bp，在98个甘草样品中发现了5个SNPs。5个SNPs中的一个为348 bp，表现为错义突变，其余为同义突变。在这5个SNPs中，348 bp是主要相关SNP，而821和848 bp是辅助相关SNP。本研究结果为GA遗传变异的分子机制提供了见解，为提高栽培甘草中GA含量和优良品种选育提供了依据。
