School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
Lipids Health Dis. 2012 Jun 13;11:74. doi: 10.1186/1476-511X-11-74.
The lipid fraction of rubber (Hevea brasiliensis (kunth. Muell)) seed was extracted and analyzed for toxicological effect. The toxicological compound such as linamarin in rubber seed oil (RSO) extracted using different solvents, such as hexane (RSOh), mixture of chloroform + methanol (RSOchl+mth) and ethanol (RSOeth) were also studied. Various methods analysis such as Fourier transforms infrared spectroscopy (FTIR) and colorimetric methods were carried out to determine the present of such compounds.
FTIR spectrum of RSO did not show any presence of cyanide peak. The determination of cyanide by using colorimetric method was demonstrated no response of the cyanide in RSO and didn't show any colored comparing with commercial cyanide which observed blue color. The results showed that no functional groups such as cyanide (C ≡ N) associated with linamarin were observed. Toxicological test using rats was also conducted to further confirm the absence of such compounds. RSO did not show any toxic potential to the rats. Bioassay experiments using shrimps had been used as test organisms to evaluate the toxicity of linamarin extract from RSO(h,) RSO(chl+mth) and RSO(eth) and LC50 were found to be (211.70 %, 139.40 %, and 117.41 %, respectively).
This can be attributed no hazardous linamarin were found in RSO.
从橡胶（巴西橡胶树（kunth. Muell））种子中提取脂类成分，并分析其毒理学效应。还研究了使用不同溶剂（例如己烷（RSOh）、氯仿+甲醇混合物（RSOchl+mth）和乙醇（RSOeth））提取的橡胶籽油（RSO）中的毒理学化合物，如亚麻氰苷。进行了各种分析方法，如傅里叶变换红外光谱（FTIR）和比色法，以确定这些化合物的存在。
RSO 的 FTIR 光谱没有显示任何氰化物峰的存在。使用比色法测定氰化物的结果表明，RSO 中的氰化物没有反应，并且与观察到蓝色的商业氰化物相比没有显示任何颜色。结果表明，没有观察到与亚麻氰苷相关的氰化物（C≡N）等官能团。还对大鼠进行了毒理学测试，以进一步证实这些化合物的不存在。RSO 对大鼠没有表现出任何潜在毒性。还使用虾作为测试生物，对 RSO（h）、RSO（chl+mth）和 RSO（eth）中亚麻氰苷提取物的毒性进行了生物测定实验，发现 LC50 分别为（211.70%、139.40%和 117.41%）。
这可以归因于在 RSO 中没有发现危险的亚麻氰苷。
