Inserm, U972, Hôpital Paul Brousse, Villejuif, France.
Macromol Biosci. 2012 Jul;12(7):901-10. doi: 10.1002/mabi.201100431. Epub 2012 Jun 13.
Human ECFCs contribute to vascular repair. For this reason, they are considered as valuable cell therapy products in ischemic diseases. Porous scaffolds are prepared that are composed of natural polysaccharides, pullulan and dextran, by chemical crosslinking without use of organic solvents. These porous scaffolds, which have pores with an average size of 42 µm and a porosity of 21%, preserve the viability and the proliferation of cord-blood ECFCs. After 7 d of culture in porous scaffolds, ECFCs express endothelial markers (CD31 and vWf) and maintain endothelial functions. The cultured cells can be easily retrieved by enzymatic degradation of the porous scaffolds. In vitro results suggest that the porous scaffold could allow cell delivery of ECFCs for treatment of vascular diseases.
人内皮祖细胞有助于血管修复。因此，它们被认为是缺血性疾病中很有价值的细胞治疗产品。通过化学交联制备由天然多糖、普鲁兰多糖和葡聚糖组成的多孔支架，而不使用有机溶剂。这些多孔支架的孔平均大小为 42μm，孔隙率为 21%，保留了脐血内皮祖细胞的活力和增殖。在多孔支架中培养 7 天后，内皮祖细胞表达内皮标记物（CD31 和 vWf）并保持内皮功能。通过多孔支架的酶降解可以很容易地回收培养的细胞。体外结果表明，多孔支架可用于内皮祖细胞的细胞递送，以治疗血管疾病。
