Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
J Virol. 2012 Aug;86(16):8821-34. doi: 10.1128/JVI.00907-12. Epub 2012 Jun 13.
The 331-kbp chlorovirus Paramecium bursaria chlorella virus 1 (PBCV-1) genome was resequenced and annotated to correct errors in the original 15-year-old sequence; 40 codons was considered the minimum protein size of an open reading frame. PBCV-1 has 416 predicted protein-encoding sequences and 11 tRNAs. A proteome analysis was also conducted on highly purified PBCV-1 virions using two mass spectrometry-based protocols. The mass spectrometry-derived data were compared to PBCV-1 and its host Chlorella variabilis NC64A predicted proteomes. Combined, these analyses revealed 148 unique virus-encoded proteins associated with the virion (about 35% of the coding capacity of the virus) and 1 host protein. Some of these proteins appear to be structural/architectural, whereas others have enzymatic, chromatin modification, and signal transduction functions. Most (106) of the proteins have no known function or homologs in the existing gene databases except as orthologs with proteins of other chloroviruses, phycodnaviruses, and nuclear-cytoplasmic large DNA viruses. The genes encoding these proteins are dispersed throughout the virus genome, and most are transcribed late or early-late in the infection cycle, which is consistent with virion morphogenesis.
噬藻体 Paramecium bursaria chlorella virus 1（PBCV-1）的基因组有 331kbp，对其进行了重新测序和注释，以纠正 15 年前原始序列中的错误；40 个密码子被认为是开放阅读框的最小蛋白大小。PBCV-1 有 416 个预测的蛋白编码序列和 11 个 tRNA。还使用两种基于质谱的方案对高度纯化的 PBCV-1 病毒粒子进行了蛋白质组分析。将质谱衍生的数据与 PBCV-1 及其宿主 Chlorella variabilis NC64A 的预测蛋白质组进行了比较。综合分析结果显示，有 148 种独特的病毒编码蛋白与病毒粒子有关（约占病毒编码能力的 35%），还有 1 种宿主蛋白。其中一些蛋白似乎具有结构/架构功能，而其他蛋白则具有酶、染色质修饰和信号转导功能。除了与其他噬藻体、藻病毒和核质大 DNA 病毒的蛋白具有同源性外，这些蛋白中的大多数（106 种）在现有的基因数据库中没有已知的功能或同源物。这些蛋白的基因散布在整个病毒基因组中，大多数在感染周期的晚期或早期晚期转录，这与病毒粒子形态发生一致。
