Institute of Aquaculture, Hellenic Centre for Marine Research (HCMR), Aghios Kosmas, 16777 Ellinikon, Athens, Greece.
Chemosphere. 2012 Nov;89(9):1091-7. doi: 10.1016/j.chemosphere.2012.05.075. Epub 2012 Jun 12.
We studied if the levels of copper released from antifouling treated nets used in finfish mariculture could affect the immune defense mechanism and/or induce oxidative stress in Dicentrarchus labrax, after short term exposure in laboratory experiments. Dissolved copper concentration released from the treated nets, copper bioavailability and a set of biomarkers responses were measured. Biomarkers included hemoglobin concentration, activities of lysozyme, total complement, respiratory burst, glutathione S-transferase and acetycholinesterase and concentration of thiobarbituric acid reactive substances. Results indicated elevated copper concentration in seawater (184 μg L(-1)) but low concentration in muscle (1.5 μg g(-1)) and liver (117 μg g(-1)). Copper bioavailability was independent of copper complexes with dissolved organic carbon. However, formation of copper complexes with other matrices could neither be excluded nor justified. The released copper from the treated nets did not induce oxidative stress but affected the immediate immune defense mechanism of the exposed fish making them more easily vulnerable to diseases. Consequently, copper-based antifouling treated nets could be a risk factor for D. labrax health.
我们研究了在短期实验室暴露后，来自水产养殖用防污处理网的释放的铜水平是否会影响斜带石斑鱼的免疫防御机制和/或诱导氧化应激。测量了从处理过的网中释放的溶解铜浓度、铜的生物利用度和一系列生物标志物的反应。生物标志物包括血红蛋白浓度、溶菌酶、总补体、呼吸爆发、谷胱甘肽 S-转移酶和乙酰胆碱酯酶的活性以及硫代巴比妥酸反应物质的浓度。结果表明，海水中的铜浓度升高（184μg/L），但肌肉（1.5μg/g）和肝脏（117μg/g）中的铜浓度较低。铜的生物利用度与溶解态有机碳中的铜络合物无关。然而，不能排除也不能证明与其他基质形成铜络合物的可能性。处理过的网中释放的铜不会引起氧化应激，但会影响暴露鱼的即时免疫防御机制，使它们更容易受到疾病的影响。因此，基于铜的防污处理网可能是斜带石斑鱼健康的一个风险因素。
