Department of Environmental Medicine, Medical Faculty, University of Rijeka, Braće Branchetta 20a, 51000, Rijeka, Croatia,
Environ Monit Assess. 2013 Nov;185(11):9009-18. doi: 10.1007/s10661-013-3230-3. Epub 2013 May 5.
The present study investigates the response of three hepatic biomarkers in adult sea bass (Dicentrarchus labrax, Linnaeus 1758) caged at a wastewater outlet of an oil refinery with fish caged at a pristine site used as controls. The biomarkers that were investigated were the hepatosomatic index (HSI), 7-ethoxyresorufin-O-deethylase (EROD) activity and glutathione-S-transferase (GST) activity. In addition, we have measured the levels of polycyclic aromatic hydrocarbons (PAHs) and selected heavy metals (lead, cadmium, mercury, copper and zinc) in sediment samples at the polluted site. Although the polluted site had high environmental levels of PAHs and heavy metals, there was no difference in hepatic EROD activity and HSI between fish caged at the polluted site and controls. On the other hand, GST activity was significantly lower in fish caged at the polluted site compared to controls. Our results point out that the studied biomarkers have limited use in environmental risk assessment studies, at least when caged adult sea bass is used as the sentinel species and complex toxicant mixtures are involved.
本研究调查了在炼油厂废水出口处笼养的成年鲈鱼（Dicentrarchus labrax，Linnaeus 1758）与在原始地点作为对照的笼养鱼类的三种肝生物标志物的反应。所研究的生物标志物是肝体比（HSI）、7-乙氧基香豆素-O-脱乙基酶（EROD）活性和谷胱甘肽-S-转移酶（GST）活性。此外，我们还测量了污染地点沉积物样本中多环芳烃（PAHs）和选定重金属（铅、镉、汞、铜和锌）的水平。尽管污染地点的 PAHs 和重金属环境水平很高，但在污染地点和对照的笼养鱼类之间，肝 EROD 活性和 HSI 没有差异。另一方面，与对照组相比，在污染地点笼养的鱼类中 GST 活性显著降低。我们的研究结果表明，在所研究的生物标志物在环境风险评估研究中具有有限的用途，至少当使用笼养成年鲈鱼作为指示物种且涉及复杂的有毒混合物时是如此。
