McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA.
Acta Biomater. 2012 Oct;8(10):3687-94. doi: 10.1016/j.actbio.2012.06.005. Epub 2012 Jun 12.
Few therapeutic options exist for meniscus repair after injury. Local delivery of growth factors may stimulate repair and create a favorable environment for engineered replacement materials. In this study we assessed the effect of basic fibroblast growth factor (bFGF) (a pro-mitotic agent) and transforming growth factor β3 (TGF-β3) (a pro-matrix formation agent) on meniscus repair and the integration/maturation of electrospun poly(ε-caprolactone) (PCL) scaffolds for meniscus tissue engineering. Circular meniscus repair constructs were formed and refilled with either native tissue or scaffolds. Repair constructs were cultured in serum-containing medium for 4 and 8weeks with various growth factor formulations, and assessed for mechanical strength, biochemical content, and histological appearance. Results showed that either short-term delivery of bFGF or sustained delivery of TGF-β3 increased integration strength for both juvenile and adult bovine tissue, with similar findings for engineered materials. While TGF-β3 increased proteoglycan content in the explants, bFGF did not increase DNA content after 8weeks of culture. This work suggests that in vivo delivery of bFGF or TGF-β3 may stimulate meniscus repair, but that the time course of delivery will strongly influence success. Further, this study demonstrates that electrospun scaffolds are a promising material for meniscus tissue engineering, achieving comparable or superior integration compared with native tissue.
对于损伤后的半月板修复,目前治疗选择有限。局部递送生长因子可能会刺激修复,并为工程替代材料创造有利的环境。在这项研究中,我们评估了碱性成纤维细胞生长因子(bFGF)(促有丝分裂剂)和转化生长因子β3(TGF-β3)(促基质形成剂)对半月板修复和静电纺聚己内酯(PCL)支架整合/成熟的影响用于半月板组织工程。形成了圆形半月板修复结构,并将其用天然组织或支架填充。修复结构在含血清的培养基中培养 4 和 8 周,采用不同的生长因子配方,评估力学强度、生化含量和组织学外观。结果表明,无论是短期 bFGF 给药还是持续 TGF-β3 给药,都能提高幼年和成年牛组织的整合强度,对工程材料也有类似的发现。虽然 TGF-β3 增加了外植体中的蛋白聚糖含量,但 bFGF 在培养 8 周后并未增加 DNA 含量。这项工作表明,体内递送 bFGF 或 TGF-β3 可能会刺激半月板修复,但递送的时间过程将强烈影响修复的效果。此外,本研究表明,静电纺丝支架是一种有前途的半月板组织工程材料,与天然组织相比,它可以实现类似或更好的整合。