Bochyńska Agnieszka Izabela, Hannink Gerjon, Verhoeven Renate, Grijpma Dirk W, Buma Pieter
Orthopaedic Research Laboratory, Department of Orthopaedics, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
MIRA Institute for Biomedical Engineering and Technical Medicine, Department of Biomaterials Science and Technology, University of Twente, Enschede, The Netherlands.
J Mater Sci Mater Med. 2017 Jan;28(1):22. doi: 10.1007/s10856-016-5832-0. Epub 2016 Dec 26.
The aim of the current in vitro study was to investigate if tissue surface modification with collagenase and addition of the TGF-β3 can increase the number of cells present in meniscus tears repaired with the use of newly developed tissue adhesives based on isocyanate-terminated block copolymers. Cylindrical explants were harvested from the inner part of bovine menisci. To simulate a full-thickness tear, the central core of the explants was removed and glued back into the defect, with or without incubation in collagenase solution prior to gluing. The repair constructs were then cultured with or without addition of TGF-β3, and assessed for their histological appearance. The histological staining of the constructs confirmed that both developed adhesives were not cytotoxic. After 28 days, meniscus cells were present in direct contact with the glues. The addition of TGF-β3 to the culture medium resulted in the presence of cells that formed a sheath inside the simulated tear and in increased cell numbers at the edges of annulus of the explants. In the group in which the tissue was incubated in collagenase and cultured in medium containing TGF-β3, thicker layers of cells were observed. These results suggest that repairing the torn meniscus with tissue adhesives after pre-treatment of the tissue with collagenase and stimulation with TGF-β3 is a very promising treatment method, especially when treating the inner avascular part of the meniscus. Nevertheless, longer-term in vitro and in vivo studies are needed to confirm the beneficial effects of this combination therapy.
当前的体外研究旨在调查，使用基于异氰酸酯封端的嵌段共聚物的新开发组织粘合剂修复半月板撕裂时，用胶原酶进行组织表面改性并添加转化生长因子-β3（TGF-β3）是否能增加存在的细胞数量。从牛半月板内部获取圆柱形外植体。为模拟全层撕裂，去除外植体的中央核心，然后在粘合前将其放回缺损处，粘合前外植体在胶原酶溶液中孵育或不孵育。然后在添加或不添加TGF-β3的情况下培养修复构建体，并评估其组织学外观。构建体的组织学染色证实两种开发的粘合剂均无细胞毒性。28天后，半月板细胞与粘合剂直接接触。向培养基中添加TGF-β3导致细胞形成鞘包裹在模拟撕裂内部，并且外植体环边缘的细胞数量增加。在组织在胶原酶中孵育并在含有TGF-β3的培养基中培养的组中，观察到细胞层更厚。这些结果表明，在用胶原酶预处理组织并使用TGF-β3刺激后，用组织粘合剂修复撕裂的半月板是一种非常有前景的治疗方法，尤其是在治疗半月板内部无血管部分时。然而，需要进行长期的体外和体内研究来证实这种联合治疗的有益效果。
