Sadowska Grazyna B, Threlkeld Steven W, Flangini Alexia, Sharma Surendra, Stonestreet Barbara S
Department of Pediatrics, Women & Infants Hospital of Rhode Island, The Alpert Medical School of Brown University, 101 Dudley Street, Providence, RI 02905, USA.
Int J Dev Neurosci. 2012 Oct;30(6):457-63. doi: 10.1016/j.ijdevneu.2012.06.001. Epub 2012 Jun 12.
Interleukin (IL)-1β and IL-6 have been implicated in brain development, injury progression, and fetal/maternal immune interactions. We examined IL-1β and IL-6 protein expression in cerebral cortex (CC) and white matter (WM) from non-ischemic ovine fetuses at 87-90, 122-127, and 135-137 days of gestation, pregnant ewes at 87-90 and 135-137 days of gestation, and fetuses exposed to 48 or 72h of reperfusion after ischemia. Protein expression was determined by Western immunoblot. In non-ischemic CC, IL-1β was higher (P<0.05) in adult sheep and fetuses at 135-137 than 87-90 and 122-127 days, and IL-6 higher at 122-127 than 87-90 days, and in adults than fetuses at 87-90, 122-127, and 135-137 days of gestation. In non-ischemic fetal WM, IL-6 was higher at 135-137 than 87-90 days, but IL-1β did not differ. In CC, IL-1β was higher in ewes at 135-137 than 87-90 days and IL-6 at 135-137 days and in non-pregnant adults than ewes at 87-90 days of gestation. In WM, IL-1β was higher in ewes at 135-137 than 87-90 days of gestation, but IL-6 did not differ. Forty-eight and 72h after ischemia, CC IL-1β was higher than in non-ischemic fetuses. Seventy-two hours after ischemia, IL-1β and IL-6 were higher in WM than CC. In conclusion, IL-1β and IL-6 exhibit developmental regulation in fetal brain, change during gestation in brains of pregnant ewes, show regional differences in normal brains of fetuses and ewes, demonstrate differential responses after ischemia in CC and WM, and IL-1β but not IL-6 increases after ischemia in CC.
白细胞介素（IL）-1β和IL-6与脑发育、损伤进展以及胎儿/母体免疫相互作用有关。我们检测了妊娠87 - 90天、122 - 127天和135 - 137天的非缺血性绵羊胎儿、妊娠87 - 90天和135 - 137天的怀孕母羊以及缺血后再灌注48或72小时的胎儿大脑皮质（CC）和白质（WM）中IL-1β和IL-6蛋白的表达。通过蛋白质免疫印迹法测定蛋白表达。在非缺血性CC中，成年绵羊和135 - 137天的胎儿IL-1β高于87 - 90天和122 - 127天的胎儿（P<0.05），122 - 127天的胎儿IL-6高于87 - 90天的胎儿，且成年绵羊高于妊娠87 - 90天、122 - 127天和135 - 137天的胎儿。在非缺血性胎儿WM中，135 - 137天的胎儿IL-6高于87 - 90天的胎儿，但IL-1β无差异。在CC中，135 - 137天的母羊IL-1β高于87 - 90天的母羊，135 - 137天的母羊以及非妊娠成年母羊的IL-6高于妊娠87 - 90天的母羊。在WM中，135 - 137天的母羊IL-1β高于妊娠87 - 90天的母羊，但IL-6无差异。缺血后48和72小时，CC中的IL-1β高于非缺血性胎儿。缺血后72小时，WM中的IL-1β和IL-6高于CC。总之，IL-1β和IL-6在胎儿脑中呈现发育调控，在怀孕母羊脑中随妊娠发生变化，在胎儿和母羊的正常脑中表现出区域差异，在CC和WM缺血后显示出不同反应，且CC缺血后IL-1β升高而IL-6未升高。
