Riviére A, Wilckens C, Löning T
Institute of Pathology, University of Hamburg, West Germany.
J Oral Pathol Med. 1990 Oct;19(9):408-13. doi: 10.1111/j.1600-0714.1990.tb00869.x.
Normal tongue and cervical mucosa, premalignant cervical and vulvar lesions, primaries and metastases of squamous cell carcinomas from the oral, laryngeal, cervical and vulvar mucosa were analyzed for c-erbB2 and c-myc transcription with northern-blots using 32P single-stranded RNA probes. Transcription of c-erbB2 and c-myc could be detected for almost all tissues including normal samples. A slightly enhanced transcription level was found in three cervical intraepithelial neoplasias of Grade III (CIN III) but in none of the malignant lesions. Increased transcription of c-myc was observed in premalignancies and malignancies. It was more frequent in oral and laryngeal squamous cell carcinomas (SCC) (8 of 9 cases) than in genital SCC (3 of 11 cases) or premalignancies (3 CIS of 14 CIN/VIN). No relationships of c-myc enhanced transcription level with tumor grading and staging were noticed. Thus, mere oncogene expression is a widespread phenomenon in tissues of different histogenesis and quantitative analysis is necessary prior to ascribe any diagnostic or prognostic relevance. Moreover, the frequency of tumors with enhanced transcription may vary for phenotypically closely related tumors of different organs.
使用32P单链RNA探针，通过Northern印迹法对正常舌和宫颈黏膜、宫颈和外阴癌前病变、口腔、喉、宫颈和外阴黏膜鳞状细胞癌的原发灶和转移灶进行c-erbB2和c-myc转录分析。几乎所有组织（包括正常样本）均可检测到c-erbB2和c-myc的转录。在3例III级宫颈上皮内瘤变（CIN III）中发现转录水平略有升高，但在恶性病变中均未发现。在癌前病变和恶性肿瘤中均观察到c-myc转录增加。在口腔和喉鳞状细胞癌（SCC）（9例中的8例）中比在生殖器SCC（11例中的3例）或癌前病变（14例CIN/VIN中的3例CIS）中更常见。未发现c-myc转录增强水平与肿瘤分级和分期之间的关系。因此，单纯的癌基因表达在不同组织发生的组织中是一种普遍现象，在赋予任何诊断或预后相关性之前进行定量分析是必要的。此外，转录增强的肿瘤频率在不同器官的表型密切相关的肿瘤中可能有所不同。
